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Default Secondary structure of human granulocyte colony-stimulating factor derived from NMR s

Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.

Related Articles Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.

FEBS Lett. 1992 Dec 21;314(3):435-9

Authors: Zink T, Ross A, Ambrosius D, Rudolph R, Holak TA

Recombinant 15N-, 13C-labeled human granulocyte colony-stimulating factor (rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein as well as residue-specific 15N-labeled samples. Assignment of the 1H, 15N backbone, and 60% 1H sidechain resonances has enabled the determination of the secondary structure of the protein. The secondary structure is dominated by alpha-helical regions with four stretches of helices between residues 11-41, 71-95, 102-124 and 144-170.

PMID: 1281794 [PubMed - indexed for MEDLINE]



Source: PubMed
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