Quinolizidine alkaloids (QAs) are toxic secondary metabolites of the Lupinus species, the presence of which limits the expansion of lupin beans consumption, despite their high protein content. Evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. However, quantitation of QAs is complicated by the fact that not all important alkaloids used for quantitation are commercially available. In this context, we developed a method for the simultaneous...
NMR-based fragment screening and lead discovery accelerated by principal component analysis
NMR-based fragment screening and lead discovery accelerated by principal component analysis
Abstract
Protein-based NMR spectroscopy has proven to be a very robust method for finding fragment leads to protein targets. However, one limitation of protein-based NMR is that the data acquisition and analysis can be time consuming. In order to streamline the scoring of protein-based NMR fragment screening data and the determination of ligand affinities using 2D NMR experiments we have developed a data analysis workflow based on principal component...
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02-29-2020 09:52 PM
Thermosetting polymer for dynamic nuclear polarization: Solidification of an epoxy resin mixture including TEMPO
From The DNP-NMR Blog:
Thermosetting polymer for dynamic nuclear polarization: Solidification of an epoxy resin mixture including TEMPO
Noda, Y., et al., Thermosetting polymer for dynamic nuclear polarization: Solidification of an epoxy resin mixture including TEMPO. Nucl. Instrum. Methods Phys. Res., Sect. A, 2015. 776(0): p. 8-14.
http://dx.doi.org/10.1016/j.nima.2014.11.114
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05-15-2015 08:02 PM
The Right Resin Means A Purer Protein - Genetic Engineering News
The Right Resin Means A Purer Protein - Genetic Engineering News
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The Right Resin Means A Purer Protein
Genetic Engineering News
Steven Cramer, Ph.D., a professor of chemical and biological engineering at Rensselaer Polytechnic Institute, is using biophysical tools (NMR, SPR, and AFM), molecular simulations, coarse-grain modeling, and â??a lot of chromatographyâ?? to develop models ...
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04-15-2014 11:00 PM
An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
Abstract The in vivo incorporation of unnatural amino acids into proteins is a well-established technique requiring an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is incorporated at a position encoded by a TAG amber codon. Although this technology provides unique opportunities to engineer protein structures, poor protein yields are usually obtained in deuterated media, hampering its application in the protein NMR...
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02-21-2012 03:40 AM
Conformational Study of 9-Dehydro-9-Trifluoromethyl Cinchona Alkaloids via 19F NMR Spectroscopy: Emergence of Trifluoromethyl Moiety as a Conformational Stabilizer and a Probe
Conformational Study of 9-Dehydro-9-Trifluoromethyl Cinchona Alkaloids via 19F NMR Spectroscopy: Emergence of Trifluoromethyl Moiety as a Conformational Stabilizer and a Probe
G. K. Surya Prakash, Fang Wang, Chuanfa Ni, Jingguo Shen, Ralf Haiges, Andrei K. Yudin, Thomas Mathew and George A. Olah
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja202373d/aop/images/medium/ja-2011-02373d_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja202373d
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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06-14-2011 02:30 AM
[NMR paper] Cell-free protein production and labeling protocol for NMR-based structural proteomic
Cell-free protein production and labeling protocol for NMR-based structural proteomics.
Related Articles Cell-free protein production and labeling protocol for NMR-based structural proteomics.
Nat Methods. 2004 Nov;1(2):149-53
Authors: Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL
Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods....
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11-24-2010 10:01 PM
[NMR paper] 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents dir
1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.
Related Articles 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.
J Lipid Res. 1994 Dec;35(12):2292-304
Authors: Ala-Korpela M, Korhonen A, Keisala J, Hörkkö S, Korpi P, Ingman LP, Jokisaari J, Savolainen MJ, Kesäniemi YA
A new method is presented for absolute quantitation of lipid and protein contents of human lipoproteins directly from plasma. The method enables complete lipoprotein...
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08-22-2010 03:29 AM
[NMR analysis blog] Basis on qNMR: Intramolecular vs Mixtures qNMR
Basis on qNMR: Intramolecular vs Mixtures qNMR
A bit of historical background
NMR has won its reputation as a powerful tool for structure determination of organic molecules. In addition to the information provided by chemical shifts and coupling constants, the quantitative relationships existing between the peaks (or groups of peaks - multiplets) arising from the various nuclides in the sample has proven pivotal for the assignment and interpretation of NMR spectra.
Despite the fact that the concept of quantitative NMR (qNMR) has been coupled to NMR since the early 1950, shortly...
Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol
Linear Mode
