l-asparaginase II (MW 135 kDa) from E. coli is an FDA-approved protein drug used for the treatment of childhood leukemia. Despite its long history as a chemotherapeutic, the structural basis of enzyme action, in solution, remains widely contested. In this work, methyl-based 2D [¹H-^(13)C]-heteronuclear single-quantum correlation (HSQC) NMR, at natural abundance, has been used to profile the enzymatic activity of the commercially available enzyme drug. The [¹H-^(13)C]-HSQC NMR spectra of the...
[ASAP] Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance
Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance
Rachayita Nag, Srishti Joshi, Anurag Singh Rathore, and Subhabrata Majumder
https://pubs.acs.org/cms/10.1021/jacs.3c02154/asset/images/medium/ja3c02154_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.3c02154
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Journal Highlight: Authentication of the origin of sucrose-based sugar products using quantitative natural abundance 13C NMR
Journal Highlight: Authentication of the origin of sucrose-based sugar products using quantitative natural abundance 13C NMR
http://www.spectroscopynow.com/common/images/thumbnails/154eca4d25d.jpgA direct 13C nuclear magnetic resonance (NMR) method can differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant), providing an alternative to isotope ratio MS.
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[NMR paper] Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
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Anal Chem. 2015 Apr 7;87(7):3556-61
Authors: Arbogast LW, Brinson RG, Marino JP
Abstract
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding...
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[NMR paper] NMR profiling of biomolecules at natural abundance using 2D (1)H-(15)N and (1)H-(13)C multiplicity-separated (MS) HSQC spectra.
NMR profiling of biomolecules at natural abundance using 2D (1)H-(15)N and (1)H-(13)C multiplicity-separated (MS) HSQC spectra.
NMR profiling of biomolecules at natural abundance using 2D (1)H-(15)N and (1)H-(13)C multiplicity-separated (MS) HSQC spectra.
J Magn Reson. 2014 Dec 4;251C:65-70
Authors: Chen K, Freedberg DI, Keire DA
Abstract
2D NMR (1)H-X (X=(15)N or (13)C) HSQC spectra contain cross-peaks for all XHn moieties. Multiplicity-edited(1)H-(13)C HSQC pulse sequences generate opposite signs between peaks of CH2 and...
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[NMR paper] NMR profiling of biomolecules at natural abundance using 2D 1H-15N and 1H-13C multiplicity-separated (MS) HSQC spectra
NMR profiling of biomolecules at natural abundance using 2D 1H-15N and 1H-13C multiplicity-separated (MS) HSQC spectra
Publication date: Available online 4 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Kang Chen , Darón I. Freedberg , David A. Keire</br>
2D NMR 1H-X (X=15N or 13C) HSQC spectra contain cross-peaks for all XHn moieties. Multiplicity-edited 1H-13C HSQC pulse sequences generate opposite signs between peaks of CH2 and CH/CH3 at a cost of lower signal-to-noise due to the 13C T2 relaxation during an additional 1/1 J CH period. Such...
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Journal Highlight: 1H and 13C NMR-based sugar profiling with chemometric analysis and antioxidant activity of herbhoneys and honeys
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[NMR paper] Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected he
Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
Related Articles Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
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Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance
Linear Mode
