BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 04-03-2020, 09:41 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 20,430
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Phosphate-silica interactions in diatom biosilica and synthetic composites studied by Rotational Echo Double Resonance (REDOR) NMR spectroscopy.

Phosphate-silica interactions in diatom biosilica and synthetic composites studied by Rotational Echo Double Resonance (REDOR) NMR spectroscopy.

Related Articles Phosphate-silica interactions in diatom biosilica and synthetic composites studied by Rotational Echo Double Resonance (REDOR) NMR spectroscopy.

Langmuir. 2020 Apr 01;:

Authors: Kolbe F, Daus F, Geyer A, Brunner E

Abstract
Biosilica is a biogenic composite material produced by organisms like diatoms. Various biomolecules are tightly attached or incorporated into biosilica. Examples are special proteins termed silaffins and long-chain polyamines (LCPAs). Presumably, these biomolecules are involved in the biosilica formation process. Silaffins are highly phosphorylated zwitterions with LCPAs post-translationally attached to lysine residues. In the present work, we use distance-dependent solid-state NMR experiments, especially the 31P{29Si} Rotational Echo Double Resonance (REDOR) technique, to study the environment of phosphate moieties in biosilica and in vitro synthesized SiO2-based composites. In contrast to the heterogeneous mixtures of biomolecules found in native biosilica, the described in vitro silicification experiments make use of a single synthetic phosphopeptide and a LCPA of well-defined and uniform structure. The heteronuclear correlations measured from these silica composites provide reliable 31P-29Si dipolar second moments and information about the distribution of the phosphopeptide within the silica material. The calculated second moment indicates close contact between phosphopeptides and silica. The phosphopeptides are incorporated into the silica composite in a disperse manner. Moreover, the REDOR data acquired for diatom biosilica also imply that phosphate groups are part of the silica-organic interface in this material.


PMID: 32233513 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] PSCD Domains of Pleuralin-1 from the Diatom Cylindrotheca fusiformis: NMR Structures and Interactions with Other Biosilica-Associated Proteins.
PSCD Domains of Pleuralin-1 from the Diatom Cylindrotheca fusiformis: NMR Structures and Interactions with Other Biosilica-Associated Proteins. PSCD Domains of Pleuralin-1 from the Diatom Cylindrotheca fusiformis: NMR Structures and Interactions with Other Biosilica-Associated Proteins. Structure. 2016 Jun 14; Authors: De Sanctis S, Wenzler M, Kröger N, Malloni WM, Sumper M, Deutzmann R, Zadravec P, Brunner E, Kremer W, Kalbitzer HR Abstract Diatoms are eukaryotic unicellular algae characterized by silica cell walls and...
nmrlearner Journal club 0 06-21-2016 01:30 PM
PSCD Domains of Pleuralin-1 from the Diatom Cylindrotheca fusiformis: NMR Structures and Interactions with Other Biosilica-Associated Proteins
PSCD Domains of Pleuralin-1 from the Diatom Cylindrotheca fusiformis: NMR Structures and Interactions with Other Biosilica-Associated Proteins Publication date: Available online 16 June 2016 Source:Structure</br> Author(s): Silvia De*Sanctis, Michael Wenzler, Nils Kröger, Wilhelm*M. Malloni, Manfred Sumper, Rainer Deutzmann, Patrick Zadravec, Eike Brunner, Werner Kremer, Hans*Robert Kalbitzer</br> Diatoms are eukaryotic unicellular algae characterized by silica cell walls and associated with three unique protein families, the pleuralins,...
nmrlearner Journal club 0 06-17-2016 12:06 AM
Towarda Biorelevant Structure of Protein Kinase CBound Modulators: Design, Synthesis, and Evaluation of Labeled BryostatinAnalogues for Analysis with Rotational Echo Double Resonance NMR Spectroscopy
Towarda Biorelevant Structure of Protein Kinase CBound Modulators: Design, Synthesis, and Evaluation of Labeled BryostatinAnalogues for Analysis with Rotational Echo Double Resonance NMR Spectroscopy Brian A. Loy, Adam B. Lesser, Daryl Staveness, Kelvin L. Billingsley, Lynette Cegelski and Paul A. Wender http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b00886/20150304/images/medium/ja-2015-00886q_0011.gif Journal of the American Chemical Society DOI: 10.1021/jacs.5b00886 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
nmrlearner Journal club 0 03-04-2015 08:57 PM
[NMR paper] Staphylococcus aureus peptidoglycan stem packing by rotational-echo double resonance NMR spectroscopy.
Staphylococcus aureus peptidoglycan stem packing by rotational-echo double resonance NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Staphylococcus aureus peptidoglycan stem packing by rotational-echo double resonance NMR spectroscopy. Biochemistry. 2013 May 28;52(21):3651-9 Authors: Kim SJ, Singh M, Preobrazhenskaya M, Schaefer J Abstract Staphylococcus aureus grown in the presence of an alanine-racemase inhibitor was labeled with d-alanine...
nmrlearner Journal club 0 02-11-2014 09:58 PM
Staphylococcus aureus PeptidoglycanStem Packing by Rotational-Echo Double Resonance NMR Spectroscopy
Staphylococcus aureus PeptidoglycanStem Packing by Rotational-Echo Double Resonance NMR Spectroscopy http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi4005039/aop/images/medium/bi-2013-005039_0010.gif Biochemistry DOI: 10.1021/bi4005039 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/HqAMCqHoUys More...
nmrlearner Journal club 0 05-15-2013 02:51 AM
[NMR paper] Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-eno
Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase. Related Articles Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase. J Biomol NMR. 2004 Jan;28(1):11-29 Authors: McDowell LM, Poliks B, Studelska DR, O'Connor RD, Beusen DD, Schaefer J The 46-kD enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase catalyzes the condensation of shikimate-3-phosphate (S3P) and phosphoenolpyruvate to form EPSP....
nmrlearner Journal club 0 11-24-2010 09:25 PM
[NMR paper] Closed form of liganded glutamine-binding protein by rotational-echo double-resonance
Closed form of liganded glutamine-binding protein by rotational-echo double-resonance NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Closed form of liganded glutamine-binding protein by rotational-echo double-resonance NMR. Biochemistry. 1997 Aug 5;36(31):9405-8 Authors: Klug CA, Tasaki K, Tjandra N, Ho C, Schaefer J Rotational-echo double-resonance NMR has been used to determine internuclear distances in the complex of glutamine-binding protein and its ligand, l-glutamine. The...
nmrlearner Journal club 0 08-22-2010 05:08 PM
[NMR paper] Two-dimensional, rotational-echo double-resonance NMR of cell culture metabolism.
Two-dimensional, rotational-echo double-resonance NMR of cell culture metabolism. Related Articles Two-dimensional, rotational-echo double-resonance NMR of cell culture metabolism. J Biol Chem. 1993 Oct 5;268(28):20768-71 Authors: McDowell LM, Cohen ER, Schaefer J Two-dimensional, rotational-echo double-resonance 13C NMR, a new solid-state NMR technique, has been used to show that the relative fluxes of the labeled chemical bond of L-serine along four metabolic pathways (direct purine synthesis, direct glycine incorporation into protein,...
nmrlearner Journal club 0 08-22-2010 03:01 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2020, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 03:01 PM.


Map