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Default Perfluoro-tert-butyl-homoserine as a sensitive (19) F NMR reporter for peptide-membrane interactions in solution.

Perfluoro-tert-butyl-homoserine as a sensitive (19) F NMR reporter for peptide-membrane interactions in solution.

Related Articles Perfluoro-tert-butyl-homoserine as a sensitive (19) F NMR reporter for peptide-membrane interactions in solution.

J Pept Sci. 2013 Mar 19;

Authors: Buer BC, Levin BJ, Marsh EN

Abstract
Fluorine ((19) F) NMR is a valuable tool for studying dynamic biological processes. However, increasing the sensitivity of fluorinated reporter molecules is a key to reducing acquisition times and accessing transient biological interactions. Here, we evaluate the utility a novel amino acid, l-O-(perfluoro-t-butyl)-homoserine (pFtBSer), that can easily be synthesized and incorporated into peptides and provides greatly enhanced sensitivity over currently used (19) F biomolecular NMR probes. Incorporation of pFtBSer into the potent antimicrobial peptide MSI-78 results in a sharp (19) F NMR singlet that can be readily detected at concentrations of 5 Ķm and lower. We demonstrate that pFtBSer incorporation into MSI-78 provides a sensitive tool to study binding through (19) F NMR chemical shift and nuclear relaxation changes. These results establish future potential for pFtBSer to be incorporated into various proteins where NMR signal sensitivity is paramount, such as in-cell investigations. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.


PMID: 23509011 [PubMed - as supplied by publisher]



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