Processing of milk involves heating, which can modify the structure and digestibility of its proteins. In vitro models are useful for studying protein digestion. However, validating these models with in vivo data is challenging. Here, we non-invasively monitor in vitro gastric milk protein digestion by protein-water chemical exchange detected by ¹H nuclear magnetic resonance (NMR) magnetization transfer (MT). We obtained either a fitted composite exchange rate (CER) with a relative standard...
[NMR paper] Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.
Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.
J Biomol NMR. 2017 Nov 08;:
Authors: Jolly MM, Jarvis JA, Carravetta M, Levitt MH, Williamson...
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11-10-2017 01:30 AM
Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments
Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments
Abstract
Resonance assignment is the first stage towards solving the structure of a protein. This is normally achieved by the employment of separate inter and intra residue experiments. By utilising the mixed rotation and rotary recoupling (MIRROR) condition it is possible to double the information content through the efficient bidirectional transfer of magnetization from the CO to its adjacent Cα...
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11-09-2017 08:55 AM
Modeling of Polarization Transfer Kinetics in Protein Hydration Using Hyperpolarized Water
From The DNP-NMR Blog:
Modeling of Polarization Transfer Kinetics in Protein Hydration Using Hyperpolarized Water
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Kim, J., M. Liu, and C. Hilty, Modeling of Polarization Transfer Kinetics in Protein Hydration Using Hyperpolarized Water. The Journal of Physical Chemistry B, 2017. 121(27): p. 6492-6498.
http://dx.doi.org/10.1021/acs.jpcb.7b03052
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11-01-2017 07:49 PM
Metabolite release and protein hydrolysis during the in vitro digestion of cooked sea bass fillets. A study by 1H NMR
Metabolite release and protein hydrolysis during the in vitro digestion of cooked sea bass fillets. A study by 1H NMR
Publication date: Available online 18 January 2016
Source:Food Research International</br>
Author(s): Natalia P. Vidal, Gianfranco Picone, Encarnacion Goicoechea, Luca Laghi, María J. Manzanos, Francesca Danesi, Alessandra Bordoni, Francesco Capozzi, María D. Guillén</br>
Proton Nuclear Magnetic Resonance (1H NMR) was used to study micro-wave cooked European sea bass (Dicentrarchus labrax) fillets, along five different points of...
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01-19-2016 07:18 AM
[NMR paper] Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Anal Chem. 2013 Sep 12;
Authors: Yamasaki K, Obara Y, Hasegawa M, Tanaka H, Yamasaki T, Wakuda T, Okada M, Kohzuma T
Abstract
An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter...
[NMR paper] Dissection of heteronuclear NMR experiments for studies of magnetization transfer eff
Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.
Related Articles Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.
J Magn Reson. 2003 Nov;165(1):89-94
Authors: Braun D, Wüthrich K, Wider G
Modern NMR experiments for applications with biological macromolecules in solution typically include multiple magnetization transfer steps. When working with large structures, a significant fraction of the magnetization is lost during these transfers. For the design...
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11-24-2010 09:16 PM
[NMR paper] 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative mea
19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes.
Related Articles 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes.
NMR Biomed. 1994 Nov;7(7):330-8
Authors: Gilboa H, Chapman BE, Kuchel PW
The 19F NMR Ca(2+)-indicator molecule 5,5'-difluoro-1,2-bis(o-...
Non-invasive monitoring of in vitro gastric milk protein digestion kinetics by (1)H NMR magnetization transfer
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