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Default NMR of sedimented, fibrillized, silica-entrapped and microcrystalline (metallo)proteins.

NMR of sedimented, fibrillized, silica-entrapped and microcrystalline (metallo)proteins.

NMR of sedimented, fibrillized, silica-entrapped and microcrystalline (metallo)proteins.

J Magn Reson. 2015 Apr;253:60-70

Authors: Ravera E, Schubeis T, Martelli T, Fragai M, Parigi G, Luchinat C

Abstract
Resolution and sensitivity in solid state NMR (SSNMR) can rival the results achieved by solution NMR, and even outperform them in the case of large systems. However, several factors affect the spectral quality in SSNMR samples, and not all systems turn out to be equally amenable for this methodology. In this review we attempt at analyzing the causes of this variable behavior and at providing hints to increase the chances of experimental success.


PMID: 25797005 [PubMed - in process]



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