Related ArticlesNMR investigation and secondary structure of domains I and II of rat brain calbindin D28k (1-93).
Eur J Biochem. 1999 Jun;262(3):933-8
Authors: Klaus W, Grzesiek S, Labhardt AM, Buchwald P, Hunziker W, Gross MD, Kallick DA
Calbindin D28k, a member of the troponin C superfamily of calcium-binding proteins, contains six putative EF hand domains but binds only four calcium-atoms: one at a binding site of very high affinity and three calcium-atoms at binding sites of lower affinity. The high-affinity site could be located within domain I while domains III, IV, and V bind calcium less tightly. The recombinant protein construct calb I-II (residues 1-93) comprising the first two EF hands affords a unique opportunity to study a pair of EF hands with one site binding calcium tightly and the second site empty. A series of heteronuclear 2D, 3D and 4D high-resolution NMR experiments were applied to calb I-II, and led to the complete assignment of the 1H, 13C and 15N resonances. The secondary structure of the protein was deduced from the size of the 3JHN-Halpha coupling constants, the chemical shift indices of 1Etaalpha, 13Calpha, 13C' and 13Cbeta nuclei and from an analysis of backbone NOEs observed in 3D and 4D NOESY spectra. Four major alpha-helices are identified: Ala13-Phe23, Gly33-Ala50, Leu54-Asp63, Val76-Leu90, while residues Ala2-Leu6 form a fifth, flexible helical segment. Two short beta-strands (Tyr30-Glu32, Lys72-Gly74) are found preceding helices B and D and are arranged in an anti-parallel interaction. Based on these data a structural model of calb I-II was constructed that shows that the construct adopts a tertiary structure related to other well-described calcium-binding proteins of the EF-hand family. Surprisingly, the protein forms a homodimer in solution, as was shown by its NMR characterization, size-exclusion chromatography and analytical ultra-centrifugation studies.
Solution structure, dynamics and thermodynamics of the three SH3 domains of CD2AP
Solution structure, dynamics and thermodynamics of the three SH3 domains of CD2AP
Abstract CD2 associated protein (CD2AP) is an adaptor protein that plays an important role in cell to cell union needed for the kidney function. It contains three N-terminal SH3 domains that are able to interact among others with CD2, ALIX, c-Cbl and Ubiquitin. To understand the role of the individual SH3 domains of this adaptor protein we have performed a complete structural, thermodynamic and dynamic characterization of the separate domains using NMR and DSC. The energetic contributions to the stability...
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[NMR paper] Crystal structure and NMR studies of the apo SH2 domains of ZAP-70: two bikes rather
Crystal structure and NMR studies of the apo SH2 domains of ZAP-70: two bikes rather than a tandem.
Related Articles Crystal structure and NMR studies of the apo SH2 domains of ZAP-70: two bikes rather than a tandem.
Biochemistry. 2002 Dec 3;41(48):14176-84
Authors: Folmer RH, Geschwindner S, Xue Y
The protein kinase ZAP-70 is involved in T-cell activation, and interacts with tyrosine-phosphorylated peptide sequences known as immunoreceptor tyrosine activation motifs (ITAMs), which are present in three of the subunits of the T-cell receptor....
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11-24-2010 08:58 PM
[NMR paper] Structure and dynamics of MarA-DNA complexes: an NMR investigation.
Structure and dynamics of MarA-DNA complexes: an NMR investigation.
Related Articles Structure and dynamics of MarA-DNA complexes: an NMR investigation.
J Mol Biol. 2001 Nov 16;314(1):113-27
Authors: Dangi B, Pelupessey P, Martin RG, Rosner JL, Louis JM, Gronenborn AM
An unanswered question regarding gene regulation is how certain proteins are capable of binding to DNA with high affinity at specific but highly degenerate consensus sequences. We have investigated the interactions between the Escherichia coli transcription factor, MarA, and its...
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11-19-2010 08:44 PM
[NMR paper] Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR stu
Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR study of the interaction with its RNA target.
Related Articles Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR study of the interaction with its RNA target.
J Mol Biol. 2000 Oct 20;303(2):227-41
Authors: Allain FH, Gilbert DE, Bouvet P, Feigon J
Nucleolin is an abundant 70 kDa nucleolar protein involved in many aspects of ribosomal RNA biogenesis. The central region of nucleolin contains four tandem consensus RNA-binding domains...
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11-19-2010 08:29 PM
[NMR paper] Studies on solution NMR structure of brazzein : Secondary structure and molecular sca
Studies on solution NMR structure of brazzein : Secondary structure and molecular scaffold.
Related Articles Studies on solution NMR structure of brazzein : Secondary structure and molecular scaffold.
Sci China C Life Sci. 1999 Aug;42(4):409-19
Authors: Gao G, Dai J, Ding M, Hellekant G, Wang J, Wang D
Brazzein is a sweet-tasting protein isolated from the fruit of West African plantPentadiplandra brazzeana Baillon. It is the smallest and the most water-soluble sweet protein discovered so far and is highly thermostable. The proton NMR study of...
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11-18-2010 08:31 PM
[NMR paper] Primary structure, sequence-specific 1H-NMR assignments and secondary structure in so
Primary structure, sequence-specific 1H-NMR assignments and secondary structure in solution of bromelain inhibitor VI from pineapple stem.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Primary structure, sequence-specific 1H-NMR assignments and secondary structure in solution of bromelain inhibitor VI from pineapple stem.
Eur J Biochem. 1995 Sep 1;232(2):335-43
Authors: Hatano K, Kojima M, Tanokura M, Takahashi K
One of the...
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08-22-2010 03:50 AM
[NMR paper] Determination of the solution structure of Apo calbindin D9k by NMR spectroscopy.
Determination of the solution structure of Apo calbindin D9k by NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Determination of the solution structure of Apo calbindin D9k by NMR spectroscopy.
J Mol Biol. 1995 Jun 2;249(2):441-62
Authors: Skelton NJ, Kördel J, Chazin WJ
The three-dimensional structure of apo calbindin D9k has been determined using constraints generated from nuclear magnetic resonance spectroscopy. The family of solution structures...
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Solution structure of the RBD1,2 domains from human nucleolin
Solution structure of the RBD1,2 domains from human nucleolin
Content Type Journal Article
DOI 10.1007/s10858-010-9412-1
Authors
Sengodagounder Arumugam, University of Louisville JG Brown Cancer Center 505 South Hancock St. Louisville KY 40202 USA
M. Clarke Miller, University of Louisville JG Brown Cancer Center 505 South Hancock St. Louisville KY 40202 USA
James Maliekal, University of Louisville JG Brown Cancer Center 505 South Hancock St. Louisville KY 40202 USA
NMR investigation and secondary structure of domains I and II of rat brain calbindin
Linear Mode
