[NMR paper] NMR assignments and ligand-binding studies on a two-domain family GH19 chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen.
Related ArticlesNMR assignments and ligand-binding studies on a two-domain family GH19 chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen.
Biomol NMR Assign. 2017 Apr;11(1):85-90
Authors: Takashima T, Ohnuma T, Fukamizo T
Abstract
A two-domain family GH19 chitinase from Japanese cedar (Cryptomeria japonica) pollen, CJP-4, which consists of an N-terminal CBM18 domain and a GH19 catalytic domain, is known to be an important allergen, that causes pollinosis. We report here the resonance assignments of the NMR spectrum of CJP-4. The backbone resonances were almost completely assigned, and the secondary structure was estimated based on the chemical shift values. The addition of a chitin dimer to the enzyme solution perturbed the chemical shifts of the resonances of amino acid residues forming a long extended binding site spanning from the CBM18 domain to the GH19 catalytic domain.
[NMR paper] NMR resonance assignments of a hypoallergenic isoform of the major birch pollen allergen Bet v 1.
NMR resonance assignments of a hypoallergenic isoform of the major birch pollen allergen Bet v 1.
Related Articles NMR resonance assignments of a hypoallergenic isoform of the major birch pollen allergen Bet v 1.
Biomol NMR Assign. 2017 Aug 14;:
Authors: Ahammer L, Grutsch S, Wallner M, Ferreira F, Tollinger M
Abstract
In Northern America and Europe a great number of people are suffering from birch pollen allergy and pollen related food allergies. The trigger for these immunological reactions is the 17.5*kDa major birch pollen...
nmrlearner
Journal club
0
08-17-2017 01:01 PM
[NMR paper] NMR resonance assignments of the major apple allergen Mal d 1.
NMR resonance assignments of the major apple allergen Mal d 1.
Related Articles NMR resonance assignments of the major apple allergen Mal d 1.
Biomol NMR Assign. 2016 May 11;
Authors: Ahammer L, Grutsch S, Tollinger M
Abstract
The major apple allergen Mal d 1 is the predominant cause of apple (Malus domestica) allergies in large parts of Europe and Northern America. Allergic reactions against this 17.5*kDa protein are the consequence of initial sensitization to the structurally homologous major allergen from birch pollen, Bet v...
nmrlearner
Journal club
0
05-12-2016 09:30 PM
[NMR paper] Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
PLoS One. 2013;8(9):e74040
Authors: Huan X, Shi J, Lim L, Mitra S, Zhu W, Qin H, Pasquale EB, Song J
Abstract
The 16 EphA and EphB receptors represent the largest family of receptor tyrosine kinases, and their interactions...
nmrlearner
Journal club
0
10-03-2013 03:31 PM
[NMR paper] Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11).
Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11).
Related Articles Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11).
Biochem J. 2013 Jan 29;
Authors: Viegas A, Sardinha J, Freire F, Duarte DF, Carvalho AL, Fontes CM, Romão MJ, Macedo AL, Cabrita EJ
Abstract
Non-catalytic cellulosomal carbohydrate-binding modules (CBMs) are responsible for increasing the catalytic efficiency of...
[NMR paper] Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris
Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: detection of partial oxidation of methionine by NMR.
Related Articles Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: detection of partial oxidation of methionine by NMR.
Protein Expr Purif. 2004 Oct;37(2):336-43
Authors: Barral P, Tejera ML, Treviño MA, Batanero E, Villalba M, Bruix M, Rodríguez R
Olive pollen is one of the main causes of allergy in Mediterranean countries. Ole e 6, an olive pollen...
nmrlearner
Journal club
0
11-24-2010 10:01 PM
[NMR paper] NMR solution structure of Ole e 6, a major allergen from olive tree pollen.
NMR solution structure of Ole e 6, a major allergen from olive tree pollen.
Related Articles NMR solution structure of Ole e 6, a major allergen from olive tree pollen.
J Biol Chem. 2004 Sep 10;279(37):39035-41
Authors: Treviño MA, García-Mayoral MF, Barral P, Villalba M, Santoro J, Rico M, Rodríguez R, Bruix M
Ole e 6 is a pollen protein from the olive tree (Olea europaea) that exhibits allergenic activity with a high prevalence among olive-allergic individuals. The three-dimensional structure of Ole e 6 has been determined in solution by NMR...
nmrlearner
Journal club
0
11-24-2010 09:51 PM
[NMR paper] Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protei
Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protein kinases. An isolated but fully functional Gbeta binding domain from Ste20p is only partially folded as shown by heteronuclear NMR spectroscopy.
Related Articles Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protein kinases. An isolated but fully functional Gbeta binding domain from Ste20p is only partially folded as shown by heteronuclear NMR spectroscopy.
J Biol Chem. 2001 Nov 2;276(44):41205-12
Authors: Song J, Chen Z, Xu P, Gingras R,...
NMR assignments and ligand-binding studies on a two-domain family GH19 chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen.
Linear Mode
