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Default Investigating protein-ligand interactions by solution NMR spectroscopy.

Investigating protein-ligand interactions by solution NMR spectroscopy.

Investigating protein-ligand interactions by solution NMR spectroscopy.

Chemphyschem. 2018 Jan 04;:

Authors: Becker W, Bhattiprolu KC, Gubensäk N, Zangger K

Abstract
Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large repertoire of techniques to study such complexes. Here we give an overview of the different NMR approaches available. The information they provide ranges from the simple information about the presence of binding or epitope mapping to the complete 3D structure of the complex. NMR spectroscopy is particularly useful for the study of weak interactions and for the screening of binding ligands with atomic resolution.


PMID: 29314603 [PubMed - as supplied by publisher]



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