Related ArticlesInsight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Oct 28;
Authors: Jantschke A, Koers E, Mance D, Weingarth M, Brunner E, Baldus M
Abstract
Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica-associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random-coil and ?-strand conformations. Furthermore, biosilica-associated long-chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.
PMID: 26509491 [PubMed - as supplied by publisher]
[NMR paper] Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Related Articles Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Nat Methods. 2015 May 18;
Authors: Kaplan M, Cukkemane A, van Zundert GC, Narasimhan S, Daniëls M, Mance D, Waksman G, Bonvin AM, Fronzes R, Folkers GE, Baldus M
Abstract
Studying biomolecules at atomic resolution in their native environment is the ultimate aim of structural biology. We investigated the bacterial type IV secretion system core...
[NMR paper] Resolution and measurement of heteronuclear dipolar couplings of a noncrystalline protein immobilized in a biological supramolecular assembly by proton-detected MAS solid-state NMR spectroscopy
Resolution and measurement of heteronuclear dipolar couplings of a noncrystalline protein immobilized in a biological supramolecular assembly by proton-detected MAS solid-state NMR spectroscopy
Publication date: Available online 26 October 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Sang Ho Park , Chen Yang , Stanley J. Opella , Leonard J. Mueller</br>
Two-dimensional 15N chemical shift/1H chemical shift and three-dimensional 1H-15N dipolar coupling/15N chemical shift/1H chemical shift MAS solid-state NMR correlation spectra of the filamentous...
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10-27-2013 12:53 AM
[NMR paper] Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
J Phys Chem A. 2013 Jul 8;
Authors: Burgess KM, Xu Y, Leclerc MC, Bryce DL
Abstract
We report on the 25Mg solid-state nuclear magnetic resonance (NMR) characterization of a series of magnesium complexes featuring Mg2+ ions in organic coordination environments. Six compounds have...
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07-10-2013 05:30 PM
[NMR paper] Solid-State NMR-Based Approaches for Supramolecular Structure Elucidation.
Solid-State NMR-Based Approaches for Supramolecular Structure Elucidation.
Related Articles Solid-State NMR-Based Approaches for Supramolecular Structure Elucidation.
Acc Chem Res. 2013 Apr 15;
Authors: Weingarth M, Baldus M
Abstract
Supramolecular chemistry provides structural and conformational information about complexes formed from multiple molecules. While the molecule is held together by strong intramolecular contacts like covalent bonds, supramolecular structures can be further stabilized by weaker or transient intermolecular...
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04-17-2013 08:15 PM
[NMR paper] Optimizing oriented planar-supported lipid samples for solid-state protein NMR.
Optimizing oriented planar-supported lipid samples for solid-state protein NMR.
Related Articles Optimizing oriented planar-supported lipid samples for solid-state protein NMR.
Biophys J. 2005 Oct;89(4):2792-805
Authors: Rainey JK, Sykes BD
Sample orientation relative to the static magnetic field of an NMR spectrometer allows study of membrane proteins in the lipid bilayer setting. The straightforward preparation and handling of extremely thin mica substrates with consistent surface properties has prompted us to examine oriented phospholipid...
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12-01-2010 06:56 PM
Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
Related Articles Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
J Am Chem Soc. 2010 Oct 8;
Authors: Loquet A, Giller K, Becker S, Lange A
We present a robust solid-state NMR approach for the accurate determination of molecular interfaces in insoluble and noncrystalline protein-protein complexes. The method relies on the measurement of intermolecular (13)C-(13)C distances in mixtures of glucose- and glucose-labeled proteins. We have...
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Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy
Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy
Antoine Loquet, Karin Giller, Stefan Becker and Adam Lange
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107460j/aop/images/medium/ja-2010-07460j_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja107460j
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/uejHS045F6M
Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
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