Related ArticlesFlavonoid binding to a multi-drug-resistance transporter protein: an STD-NMR study.
Anal Bioanal Chem. 2004 Aug;379(7-8):1045-9
Authors: Nissler L, Gebhardt R, Berger S
Flavonoids are well known to inhibit the function of the multi-drug-resistance (mdr) transporter by interacting with their ATP binding domains. The precise orientation of these molecules inside the ATP binding pocket is still unclear. We applied the saturation transfer difference (STD) NMR technique to investigate the binding of the flavonoid luteolin and its 7-O-beta-D-glycopyranoside to the recombinant nucleotide binding domain (NBD2) of mouse-mdr. First, this NMR technique confirmed binding of both ligands to NBD2, as was determined from tryptophan fluorescence-quenching experiments. Further, the results suggest binding of both luteolin and its 7-O-beta-D-glycopyranoside by their polar groups at positions 4, 5, and 3' to the protein.
Researchers Look at Averting Drug Resistance - Drug Discovery & Development
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Researchers Look at Averting Drug Resistance
Drug Discovery & Development
Kern and her team studied the protein using nuclear magnetic resonance spectroscopy, adding a drug mimetic in order to learn how the structure EmrE was designed and how it functioned as it was transportingâ??moving the drug from the inside of the cell ...
Researchers Look at Averting Drug Resistance - Drug Discovery & Development
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02-02-2012 09:43 PM
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
J Mol Biol. 2010 Nov 25;
Authors: Pan YR, Lou YC, Seven AB, Rizo J, Chen C
The tellurium oxyanion, TeO(3)(2-), has been used in the treatment of infectious diseases caused by mycobacteria. However, many pathogenic bacteria show tellurite resistance. Several tellurite resistance genes have been identified, and these genes...
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11-30-2010 08:14 PM
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock-out mice.
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock-out mice.
Biochem Biophys Res Commun. 2010 Nov 18;
Authors: Kim IY, Jung J, Jang M, Ahn YG, Shin JH, Choi JW, Sohn MR, Shin SM, Kang DG, Lee HS, Bae YS, Ryu DH, Seong JK, Hwang GS
AHNAK is a giant protein of approximately 700 kD identified in human neuroblastomas and skin epithelial cells. Recently, we found that AHNAK knock-out (AHNAK(-/-)) mice have a strong...
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11-26-2010 05:32 PM
[NMR paper] NMR diffusion and relaxation study of drug-protein interaction.
NMR diffusion and relaxation study of drug-protein interaction.
Related Articles NMR diffusion and relaxation study of drug-protein interaction.
Spectrochim Acta A Mol Biomol Spectrosc. 1999 Aug;55A(9):1897-901
Authors: Luo RS, Liu ML, Mao XA
In this work, NMR diffusion and relaxation measurements are applied to the study of the interaction between the anti-inflammatory drug salicylate and the human serum albumin (HSA) in solutions. The self-diffusion coefficients and the spin-lattice relaxation rates of salicylate are measured as a function...
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11-18-2010 08:31 PM
[NMR paper] An NMR study of ligand binding by maltodextrin binding protein.
An NMR study of ligand binding by maltodextrin binding protein.
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Biochem Cell Biol. 1998;76(2-3):189-97
Authors: Gehring K, Zhang X, Hall J, Nikaido H, Wemmer DE
Proton NMR spectra of maltodextrin binding protein from Escherichia coli were used to monitor conformational changes that accompany ligand binding. Chemical shift changes associated with the binding of different maltodextrins to maltodextrin binding protein were studied using one-dimensional difference...
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11-17-2010 11:06 PM
[NMR paper] NMR investigation of the multidrug transporter EmrE, an integral membrane protein.
NMR investigation of the multidrug transporter EmrE, an integral membrane protein.
Related Articles NMR investigation of the multidrug transporter EmrE, an integral membrane protein.
Eur J Biochem. 1998 Jun 15;254(3):610-9
Authors: Schwaiger M, Lebendiker M, Yerushalmi H, Coles M, Gröger A, Schwarz C, Schuldiner S, Kessler H
EmrE is an Escherichia coli multidrug transport protein that confers resistance to a wide range of toxicants by active transport across the bacterial cell membrane. The highly hydrophobic polytopic integral membrane...
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11-17-2010 11:06 PM
[NMR paper] NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein
NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein-lipid micelle interactions.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein-lipid micelle interactions.
Biochemistry. 1997 Sep 2;36(35):10709-17
Authors: Xu RX, Pawelczyk T, Xia TH, Brown SC
Classical protein kinase C (PKC) family members are activated by the binding of various ligands to one of several cysteine-rich...
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08-22-2010 05:08 PM
[NMR paper] Study on the erythrocytes from myotonic dystrophy with multi-nuclear NMR.
Study on the erythrocytes from myotonic dystrophy with multi-nuclear NMR.
Related Articles Study on the erythrocytes from myotonic dystrophy with multi-nuclear NMR.
Muscle Nerve. 1991 Jan;14(1):57-63
Authors: Kuwabara T, Yuasa T, Ohno T, Yamamuro M, Miyatake T
We have studied the water permeability through membranes, the function of the Na pump, and glucose metabolism of erythrocytes of patients with myotonic muscular dystrophy (MyD) using 1H--, 23Na, and 13C-NMR techniques. A significant decrease in water permeability was recognized in the...
Flavonoid binding to a multi-drug-resistance transporter protein: an STD-NMR study.
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