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Default Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides.

Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides.

Related Articles Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides.

Org Biomol Chem. 2013 Nov 21;11(43):7611-5

Authors: Altmayer-Henzien A, Declerck V, Aitken DJ, Lescop E, Merlet D, Farjon J

Abstract
Structural investigations of peptides using NMR spectroscopy rarely include the detection of N-H···O=C and N-H···N hydrogen bonds, because the relevant heteronuclei have a low natural abundance while the small trans hydrogen bond scalar couplings reduce the sensitivity. Fast repetition NMR techniques combined with state of the art spectrometer specifications allowed the enhancement of the sensitivity for detection of hydrogen bonds at natural isotopic abundance.


PMID: 24105064 [PubMed - indexed for MEDLINE]



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