Direct Monitoring of ?-Sheet Formationin the Outer Membrane Protein TtoA Assisted by TtOmp85

		BioNMR > Educational resources > Journal club
Direct Monitoring of ?-Sheet Formationin the Outer Membrane Protein TtoA Assisted by TtOmp85

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-28-2016, 12:07 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,191

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Direct Monitoring of ?-Sheet Formationin the Outer Membrane Protein TtoA Assisted by TtOmp85

Direct Monitoring of ?-Sheet Formationin the Outer Membrane Protein TtoA Assisted by TtOmp85

Biochemistry
DOI: 10.1021/acs.biochem.6b00691

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] NMR Polypeptide Backbone Conformation of the E.coli Outer Membrane Protein W. NMR Polypeptide Backbone Conformation of the E.coli Outer Membrane Protein W. Related Articles NMR Polypeptide Backbone Conformation of the E.*coli Outer Membrane Protein W. Structure. 2014 Jul 9; Authors: Horst R, Stanczak P, Wüthrich K Abstract The outer membrane proteins (Omps) are key factors for bacterial survival and virulence. Among the Omps that have been structurally characterized either by X-ray crystallography or by NMR in solution, the crystal structure of OmpW stands out because three of its four extracellular...	nmrlearner	Journal club	0	07-16-2014 10:46 AM
NMR Polypeptide Backbone Conformation of the E.coli Outer Membrane Protein W NMR Polypeptide Backbone Conformation of the E.coli Outer Membrane Protein W Publication date: Available online 10 July 2014 Source:Structure</br> Author(s): Reto Horst , Pawel Stanczak , Kurt Wüthrich</br> The outer membrane proteins (Omps) are key factors for bacterial survival and virulence. Among the Omps that have been structurally characterized either by X-ray crystallography or by NMR in solution, the crystal structure of OmpW stands out because three of its four extracellular loops are well defined, whereas long extracellular loops in other E.*coli Omps...	nmrlearner	Journal club	0	07-10-2014 08:28 PM
Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. J Am Chem Soc. 2011 Mar 1; Authors: Renault M, Bos MP, Tommassen J, Baldus M Multidomain proteins constitute a large part of prokaryotic and eukaryotic proteomes and play fundamental roles in various physiological processes. However, their structural characterization is challenging because of their large size and...	nmrlearner	Journal club	0	03-03-2011 12:34 PM
Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA Marie Renault, Martine P. Bos, Jan Tommassen and Marc Baldus http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja109469c/aop/images/medium/ja-2010-09469c_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja109469c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/9XN1qiW-S-I	nmrlearner	Journal club	0	03-02-2011 02:01 AM
[NMR paper] Structure, dynamics and function of the outer membrane protein A (OmpA) and influenza Structure, dynamics and function of the outer membrane protein A (OmpA) and influenza hemagglutinin fusion domain in detergent micelles by solution NMR. Related Articles Structure, dynamics and function of the outer membrane protein A (OmpA) and influenza hemagglutinin fusion domain in detergent micelles by solution NMR. FEBS Lett. 2003 Nov 27;555(1):139-43 Authors: Tamm LK, Abildgaard F, Arora A, Blad H, Bushweller JH Recent progress from our laboratories to determine structures of small membrane proteins (up to 20 kDa) in detergent micelles...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Related Articles Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nat Struct Biol. 2001 Apr;8(4):334-8 Authors: Arora A, Abildgaard F, Bushweller JH, Tamm LK We have determined the three-dimensional fold of the 19 kDa (177 residues) transmembrane domain of the outer membrane protein A of Escherichia coli in dodecylphosphocholine (DPC) micelles in solution using heteronuclear NMR. The structure consists of an eight-stranded beta-barrel...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Related Articles Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2358-63 Authors: Fernández C, Adeishvili K, Wüthrich K The (2)H,(13)C,(15)N-labeled, 148-residue integral membrane protein OmpX from Escherichia coli was reconstituted with dihexanoyl phosphatidylcholine (DHPC) in mixed micelles...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Light-induced membrane protein phosphorylation in the bovine rod outer segment. A mag Light-induced membrane protein phosphorylation in the bovine rod outer segment. A magic angle spinning 31P-NMR study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Light-induced membrane protein phosphorylation in the bovine rod outer segment. A magic angle spinning 31P-NMR study. Biophys Chem. 1990 May;36(1):27-31 Authors: Albert AD, Frye JS, Yeagle PL Magic angle spinning 31P-NMR (MAS 31P-NMR) spectra of bovine rod outer segments, unphosphorylated and...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off