BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-21-2010, 11:53 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 17,578
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR

Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR spectroscopy.

Related Articles Detection of fructose-3-phosphokinase activity in intact mammalian lenses by 31P NMR spectroscopy.

J Biol Chem. 1993 Apr 15;268(11):7763-7

Authors: Lal S, Szwergold BS, Kappler F, Brown T

Recently we have identified two novel phosphorylated metabolites in the lenses of diabetic rats as sorbitol 3-phosphate (Sor-3-P) and fructose 3-phosphate (Fru-3-P). The latter compound is of particular interest since it is a potent glycating agent, which could account, at least in part, for the increased protein cross-linking and cataract formation in the lens of the diabetic rat. In order to gain insight into the mechanism of formation of these compounds, 31P NMR spectra of rat, pig, and rabbit lenses, perfused with media supplemented with glucose, fructose, or sorbitol, were acquired. Perfusion with fructose-supplemented media resulted in the production of Fru-3-P in all three species. This compound was not produced upon perfusion with fructose-deficient media. The identification of the newly synthesized material as Fru-3-P was confirmed by spiking perchloric acid extracts of the perfused lenses with synthetic Fru-3-P. Our results provide strong evidence for the existence of a fructose-3-phosphokinase in mammalian lenses. If this enzyme is present in human lenses as well, it will reinforce the hypothesis that this enzyme and its product, Fru-3-P, may play a role in diabetic cataractogenesis and that lenticular fructose-3-phosphokinase may provide another therapeutic target in the prevention and alleviation of diabetic cataracts.

PMID: 8385119 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Configurational assignment of D- and L-isovalines in intact, natural, and synthetic p
Configurational assignment of D- and L-isovalines in intact, natural, and synthetic peptides by 2D-NMR spectroscopy. Related Articles Configurational assignment of D- and L-isovalines in intact, natural, and synthetic peptides by 2D-NMR spectroscopy. Chem Biodivers. 2010 Jun;7(6):1612-24 Authors: De Zotti M, Schievano E, Mammi S, Kaptein B, Broxterman QB, Singh SB, BrŁckner H, Toniolo C We investigated, by means of 2D-NMR, the naturally occurring and chemically synthesized 16-mer integramides A and B, which belong to a group of bioactive, fungal...
nmrlearner Journal club 0 10-22-2010 04:33 PM
[NMR paper] Three-dimensional structure of the DNA-binding domain of the fructose repressor from
Three-dimensional structure of the DNA-binding domain of the fructose repressor from Escherichia coli by 1H and 15N NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Three-dimensional structure of the DNA-binding domain of the fructose repressor from Escherichia coli by 1H and 15N NMR. J Mol Biol. 1997 Jul 18;270(3):496-510 Authors: Penin F, Geourjon C, Montserret R, Böckmann A, Lesage A, Yang YS, Bonod-Bidaud C, Cortay JC, Nègre D, Cozzone AJ, Deléage G FruR is...
nmrlearner Journal club 0 08-22-2010 05:08 PM
[NMR paper] Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs.
Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs. Proteins. 1997 Feb;27(2):204-9 Authors: Neu M, Brachvogel V, Oschkinat H, Zerial M, Metcalf P Rab proteins are a family of approximately 25kD ras-related GTPases which are associated with distinct intracellular membranes where they...
nmrlearner Journal club 0 08-22-2010 03:31 PM
[NMR paper] Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs.
Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Rab7: NMR and kinetics analysis of intact and C-terminal truncated constructs. Proteins. 1997 Feb;27(2):204-9 Authors: Neu M, Brachvogel V, Oschkinat H, Zerial M, Metcalf P Rab proteins are a family of approximately 25kD ras-related GTPases which are associated with distinct intracellular membranes where they...
nmrlearner Journal club 0 08-22-2010 03:03 PM
[NMR paper] 1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells
1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles 1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells. Biophys J. 1995 Feb;68(2):681-93 Authors: Fetler BK, Simplaceanu V, Ho C Using improved selective excitation methods for protein nuclear...
nmrlearner Journal club 0 08-22-2010 03:41 AM
[NMR paper] 1H-NMR measurement of fractional dissociation of imidazole in intact animals.
1H-NMR measurement of fractional dissociation of imidazole in intact animals. Related Articles 1H-NMR measurement of fractional dissociation of imidazole in intact animals. Am J Physiol. 1994 Mar;266(3 Pt 2):R1008-15 Authors: Hitzig BM, Perng WC, Burt T, Okunieff P, Johnson DC The alphastat hypothesis states that intracellular acid-base status is regulated to maintain constancy of the fractional dissociation of intracellular protein and enzyme imidazole-histidine (alpha-imidazole). A major drawback of this theory has been the lack of a means...
nmrlearner Journal club 0 08-22-2010 03:33 AM
[NMR paper] 1H-NMR measurement of fractional dissociation of imidazole in intact animals.
1H-NMR measurement of fractional dissociation of imidazole in intact animals. Related Articles 1H-NMR measurement of fractional dissociation of imidazole in intact animals. Am J Physiol. 1994 Mar;266(3 Pt 2):R1008-15 Authors: Hitzig BM, Perng WC, Burt T, Okunieff P, Johnson DC The alphastat hypothesis states that intracellular acid-base status is regulated to maintain constancy of the fractional dissociation of intracellular protein and enzyme imidazole-histidine (alpha-imidazole). A major drawback of this theory has been the lack of a means...
nmrlearner Journal club 0 08-22-2010 03:33 AM
[NMR paper] The detection of proline isomerase activity in FK506-binding protein by two-dimension
The detection of proline isomerase activity in FK506-binding protein by two-dimensional 1H NMR exchange spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The detection of proline isomerase activity in FK506-binding protein by two-dimensional 1H NMR exchange spectroscopy. Biochem Biophys Res Commun. 1990 Aug 31;171(1):445-50 Authors: Justice RM, Kline AD, Sluka JP, Roeder WD, Rodgers GH, Roehm N, Mynderse JS 1H NMR assignments of the trans and cis isomers of...
nmrlearner Journal club 0 08-21-2010 11:04 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 04:38 PM.


Map