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Disordered proteins:
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Default On deriving spatial protein structure from NMR or X-ray diffraction data.

On deriving spatial protein structure from NMR or X-ray diffraction data.

Related Articles On deriving spatial protein structure from NMR or X-ray diffraction data.

Ciba Found Symp. 1991;161:150-9; discussion 159-66

Authors: van Gunsteren WF, Gros P, Torda AE, Berendsen HJ, van Schaik RC

During the last decade it has become possible to derive the spatial structure of small proteins in solution using multidimensional NMR spectroscopy measurements and interpreting the data in terms of a chemical atomic model. The NMR experiments generate a set of interproton distance constraints, which is subsequently used to generate spatial structures that satisfy the experimental data. Correspondingly, crystallographic least-squares and molecular dynamics refinement is routinely applied to obtain a protein structure that is compatible with the observed structure factor amplitudes. The quality of the structure obtained will depend on the number and quality of the experimental data and on the searching power of the refinement method and protocol. The potential energy annealing conformational search (PEACS) algorithm is shown to be an improvement over standard molecular dynamics search methods. The use of time-dependent distance or structure factor restraints in molecular dynamics refinement yields a much better representation of experimental information than the fixed, static restraints which have generally been used until now. Conventional structure refinement methods lead to a too static and rigid picture of a protein in solution or in the crystalline state.

PMID: 1814692 [PubMed - indexed for MEDLINE]



Source: PubMed
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