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Default Correlated motions of C???N and C α ??C β pairs in protonated and per-deuterated GB3

Correlated motions of C???N and C α ??C β pairs in protonated and per-deuterated GB3

Abstract

We investigated correlated µs-ms time scale motions of neighboring 13C???15N and 13Cα??13Cβ nuclei in both protonated and perdeuterated samples of GB3. The techniques employed, NMR relaxation due to cross-correlated chemical shift modulations, specifically target concerted changes in the isotropic chemical shifts of the two nuclei associated with spatial fluctuations. Field-dependence of the relaxation rates permits identification of the parameters defining the chemical exchange rate constant under the assumption of a two-site exchange. The time scale of motions falls into the intermediate to fast regime (with respect to the chemical shift time scale, 100??400*s??1 range) for the 13C???15N pairs and into the slow to intermediate regime for the 13Cα??13Cβ pairs (about 150*s??1). Comparison of the results obtained for protonated and deuterated GB3 suggests that deuteration has a tendency to reduce these slow scale correlated motions, especially for the 13Cα??13Cβ pairs.



Source: Journal of Biomolecular NMR
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