While low-temperature Nuclear Magnetic Resonance (NMR) holds great promise for the analysis of unstable samples and for sensitizing NMR detection, spectral broadening in frozen protein samples is a common experimental challenge. One hypothesis explaining the additional linewidth is that a variety of conformations are in rapid equilibrium at room temperature and become frozen, creating an inhomogeneous distribution at cryogenic temperatures. Here, we investigate conformational heterogeneity by...
[NMR paper] Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
While low temperature NMR holds great promise for the analysis of unstable samples and for sensitizing NMR detection, spectral broadening in frozen protein samples is a common experimental challenge. One hypothesis explaining the additional linewidth is that a variety of conformations are in rapid equilibrium at room temperature and become frozen, creating an inhomogeneous distribution at cryogenic temperatures. Here we investigate conformational heterogeneity by measuring the backbone...
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[NMR paper] Predicted and Experimental NMR Chemical Shifts at Variable Temperatures: The Effect of Protein Conformational Dynamics
Predicted and Experimental NMR Chemical Shifts at Variable Temperatures: The Effect of Protein Conformational Dynamics
NMR chemical shifts provide a sensitive probe of protein structure and dynamics. Prediction of shifts, and therefore interpretation of shifts, particularly for the frequently measured amidic ^(15) N sites, remains a tall challenge. We demonstrate that protein ^(15) N chemical shift prediction from QM/MM predictions can be improved if conformational variation is included via MD sampling, focusing on the antibiotic target, E. coli Dihydrofolate reductase (DHFR). Variations...
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
From The DNP-NMR Blog:
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
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Weber, E.M.M., et al., Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures. Phys. Chem. Chem. Phys., 2017. 19(24): p. 16087-16094.
https://www.ncbi.nlm.nih.gov/pubmed/28598474
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12-18-2017 11:19 PM
[NMR paper] NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
Magn Reson Chem. 2017 Dec 14;:
Authors: Jaiswal N, Raikwal N, Pandey H, Agarwal N, Arora A, Poluri KM, Kumar D
...
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12-15-2017 09:07 PM
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
From The DNP-NMR Blog:
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
Anisotropic relaxation effects are well know and understood in EPR spectroscopy and have long served as measures to understand the motion (libration) of paramagnetic co-factors (quinones, nitroxide radicals etc.) in biological system. In this study the authors investigate the effect of anisotropic relaxation effects in DNP experiments.
To find more about anisotropic relaxation effects studied by EPR take a look at the work by Sergei Dzuba or the Eatons:
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11-13-2017 03:03 PM
Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
From The DNP-NMR Blog:
Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
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Grüning, W.R., et al., Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures. Helvetica Chimica Acta, 2016: p. n/a-n/a.
http://dx.doi.org/10.1002/hlca.201600122
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11-19-2016 08:35 PM
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
J Phys Chem B. 2011 Aug 1;
Authors: Su Y, Hong M
A challenge in the application of solid-state NMR spectroscopy to membrane proteins and peptides is the relatively broad linewidths compared to solution NMR spectra. To understand the linewidth contributions to membrane protein spectra, we have measured the inhomogeneous and homogeneous...
Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
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