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Default Completeness of NOEs in protein structure: a statistical analysis of NMR.

Completeness of NOEs in protein structure: a statistical analysis of NMR.

Related Articles Completeness of NOEs in protein structure: a statistical analysis of NMR.

J Biomol NMR. 1999 Jun;14(2):123-32

Authors: Doreleijers JF, Raves ML, Rullmann T, Kaptein R

The completeness of experimentally observed NOE restraints of a set of 97 NMR protein structures deposited in the PDB has been assessed. Completeness is defined as the ratio of the number of experimentally observed NOEs and the number of 'expected NOEs'. A practical definition of 'expected NOEs' based on inter-proton distances in the structures up to a given cut-off distance is proposed. The average completeness for the set of 97 structures is 68, 48, and 26% up to 3, 4, and 5 A cut-off distances, respectively. For recent state-of-the-art structures these numbers are approximately 90, 75, and 45%. Almost 20% of the observed NOEs are between atoms that are further than 5 A apart in the final structures. The completeness is independent of the relative surface accessibility and does not depend strongly on residue type, secondary structure or local precision, although the number of observed NOEs in these classes varies considerably. The completeness of NOE restraints is a useful quality criterion in the course of structure refinement. The completeness per residue is more informative than the number of NOEs per residue, which makes it a useful tool to assess the quality of the NMR data set in relation to the resulting structures.

PMID: 10610141 [PubMed - indexed for MEDLINE]



Source: PubMed
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