Related ArticlesCharacterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy.
Acta Trop. 1999 Jul 30;73(2):121-33
Authors: Gupta N, Goyal N, Singha UK, Bhakuni V, Roy R, Rastogi AK
The intracellular metabolites of long-term in vitro cultured axenic amastigotes of Leishmania donovani (strain Dd8) were determined and compared with those of promastigotes and intracellular amastigotes, employing proton NMR spectroscopy. The presence of two new metabolites, i.e. betaine and beta-hydroxybutyrate were reported. Betaine was detected in all the three stages being highest in the promastigotes while beta-hydroxybutyrate could be detected only in promastigotes and axenic amastigotes. Among other metabolites, succinate and valine were found in higher quantities in intracellular amastigotes and axenic amastigotes than in promastigotes. Acetoacetate was present only in axenic and intracellular amastigotes. The comparative metabolite profile of different parasite forms reveals that axenic amastigotes seem to represent an intermediate stage between promastigotes and intracellular amastigotes in spite of their strong resemblance to intracellular amastigotes in morphology, infectivity, biochemical studies and even in the manifestation of amastigote specific A2 protein.
(1)H NMR study of monocrotaline and its metabolites in human blood.
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(1)H NMR study of monocrotaline and its metabolites in human blood.
Food Chem Toxicol. 2011 Aug 6;
Authors: Yang YC, Crowder J, Wardle NJ, Yang L, White KN, Wang ZT, Annie Bligh SW
Monocrotaline (MCT) is a naturally occurring hepatotoxic pyrrolizidine alkaloid found in plants. This investigation is aimed at furthering the understanding of the role of blood in mediating the transport of MCT and its reactive metabolites in humans. Reactions of monocrotaline and its metabolites,...
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NMR-derived models of amidopyrine and its metabolites in complexes with rabbit cytochrome P450 2B4 reveal a structural mechanism of sequential N-dealkylation.
NMR-derived models of amidopyrine and its metabolites in complexes with rabbit cytochrome P450 2B4 reveal a structural mechanism of sequential N-dealkylation.
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Biochemistry. 2011 Mar 29;50(12):2123-34
Authors: Roberts AG, Sjögren SE, Fomina N, Vu KT, Almutairi A, Halpert JR
To understand the molecular basis of sequential N-dealkylation by cytochrome P450 2B enzymes, we studied the binding of...
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Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Protein Expr Purif. 2011 May 13;
Authors: Bellot G, Pascal R, Mendre C, Urbach S, Mouillac B, Déméné H
The vasopressin type 2 (V2R) receptor belongs to the class of G-protein coupled receptors. It is mainly expressed in the membrane of kidney tubules,...
[NMR paper] NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: e
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Biochemistry. 2002 Mar 19;41(11):3596-604
Authors: Chung DA, Zuiderweg ER, Fowler CB, Soyer OS, Mosberg HI, Neubig RR
A major, unresolved question in signal transduction by G protein coupled receptors (GPCRs) is to understand how, at atomic resolution, a GPCR activates a G protein. A...
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[NMR paper] A peroxidative model of human erythrocyte intracellular Ca2+ changes with in vivo cel
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Biochim Biophys Acta. 1995 Jan 25;1270(1):52-7
Authors: Aiken NR, Galey WR, Satterlee JD
Numerous changes occur with human erythrocyte aging in vivo, including an increase in free ionic intracellular calcium concentration (i) (N.R. Aiken et al. (1992) Biochim. Biophys....
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08-22-2010 03:41 AM
[NMR paper] Estimation of the intracellular free ADP concentration by 19F NMR studies of fluorine
Estimation of the intracellular free ADP concentration by 19F NMR studies of fluorine-labeled yeast phosphoglycerate kinase in vivo.
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Biochemistry. 1993 May 11;32(18):4895-902
Authors: Williams SP, Fulton AM, Brindle KM
Yeast phosphoglycerate kinase was selectively fluorine-labeled in vivo by inducing enzyme synthesis in stationary phase cells in the presence of 5-fluorotryptophan. Inducible...
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[NMR paper] 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic ext
31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds.
Anal Biochem. 1993 Feb 15;209(1):85-94
Authors: Crans DC, Mikus M, Marshman RW
31P NMR spectroscopy was used to compare the phosphorus compound content in aqueous, acidic, and organic...
Characterization of intracellular metabolites of axenic amastigotes of Leishmania don
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