BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 07-30-2014, 10:22 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 22,204
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default In-Cell NMR and EPR Spectroscopy of Biomacromolecules.

In-Cell NMR and EPR Spectroscopy of Biomacromolecules.

In-Cell NMR and EPR Spectroscopy of Biomacromolecules.

Angew Chem Int Ed Engl. 2014 Jul 28;

Authors: Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V

Abstract
The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including superresolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.


PMID: 25070284 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] Chemical exchange in biomacromolecules: Past, present, and future
Chemical exchange in biomacromolecules: Past, present, and future Publication date: April 2014 Source:Journal of Magnetic Resonance, Volume 241</br> Author(s): Arthur G. Palmer III</br> The perspective reviews quantitative investigations of chemical exchange phenomena in proteins and other biological macromolecules using NMR spectroscopy, particularly relaxation dispersion methods. The emphasis is on techniques and applications that quantify the populations, interconversion kinetics, and structural features of sparsely populated conformational states in equilibrium...
nmrlearner Journal club 0 03-22-2014 01:28 AM
[NMR paper] NMR Spectroscopy on Domain Dynamics in Biomacromolecules.
NMR Spectroscopy on Domain Dynamics in Biomacromolecules. Related Articles NMR Spectroscopy on Domain Dynamics in Biomacromolecules. Prog Biophys Mol Biol. 2013 May 15; Authors: Shapiro YE Abstract Domain dynamics in biomacromolecules is currently an area of intense research because of its importance for understanding the huge quantity of available data relating the structure and function of proteins and nucleic acids. Control of structural flexibility is essential for the proper functioning of the biomacromolecules. Biophysical...
nmrlearner Journal club 0 05-21-2013 02:34 PM
In-cell NMR spectroscopy
In-cell NMR spectroscopy October 2011 Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br> </br> </br> </br></br>
nmrlearner Journal club 0 12-15-2012 09:51 AM
In-cell NMR spectroscopy
In-cell NMR spectroscopy October 2011 Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br> </br> </br> </br></br>
nmrlearner Journal club 0 12-01-2012 06:10 PM
In-cell NMR spectroscopy
In-cell NMR spectroscopy Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br> Andres Y. Maldonado, David S. Burz, Alexander Shekhtman</br> </br> </br></br>
nmrlearner Journal club 0 03-09-2012 09:16 AM
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 22 September 2011</br> Paul R.*Murray, David*Collison, Simon*Daff, Nicola*Austin, Ruth*Edge, ...</br> A simple design for anin situ, three-electrode spectroelectrochemical cell is reported that can be used in commercial Q- and W-band (ca. 34 and 94 GHz, respectively) electron paramagnetic resonance (EPR) spectrometers, using standard sample tubing (1.0 and 0.5 mm inner diameter, respectively) and within variable temperature cryostat systems....
nmrlearner Journal club 0 09-24-2011 06:04 AM
[NMR paper] In-cell NMR spectroscopy.
In-cell NMR spectroscopy. Related Articles In-cell NMR spectroscopy. Methods Enzymol. 2005;394:17-41 Authors: Serber Z, Corsini L, Durst F, Dötsch V The role of a protein inside a cell is determined by both its location and its conformational state. Although fluorescence techniques are widely used to determine the cellular localization of proteins in vivo, these approaches cannot provide detailed information about a protein's three-dimensional state. This gap, however, can be filled by NMR spectroscopy, which can be used to investigate both...
nmrlearner Journal club 0 11-24-2010 11:14 PM
In-Cell NMR Spectroscopy
In-Cell NMR Spectroscopy Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 17 November 2010</br> Andres Y., Maldonado , David S., Burz , Alexander, Shekhtman</br> More...
nmrlearner Journal club 0 11-18-2010 06:16 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2022, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 01:15 AM.


Map