STIMATE is an endoplasmic reticulum (ER) resident membrane protein that plays key roles in regulating calcium signaling occurring at ER-plasma membrane (PM) junctions. It is also involved in the regulation of ER-PM junction maintenance. STIMATE contains multiple putative transmembrane domains with a polybasic C tail (STIMATE-CT) that directly interacts with stromal interaction molecule 1 (STIM1) to promote STIM1 conformational switch. Here using liposome pulldown assay, we show that STIMATE-CT...
[NMR paper] Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction.
Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction.
Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction.
Oncotarget. 2018 Jan 30;9(8):8068-8080
Authors: Verma A, Jing-Song F, Finch-Edmondson ML, Velazquez-Campoy A, Balasegaran S, Sudol M, Sivaraman J
Abstract
YES-associated protein (YAP) is a major effector protein of the Hippo tumor suppressor pathway, and is phosphorylated by the...
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[NMR paper] Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Related Articles Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Methods Mol Biol. 2017;1635:109-123
Authors: Kurauskas V, Schanda P, Sounier R
Abstract
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking...
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[NMR paper] NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
Related Articles NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
FEBS Lett. 2017 May 24;:
Authors: Dantas JM, Brausemann A, Einsle O, Salgueiro CA
Abstract
Geobacter sulfurreducens is a dissimilatory metal reducing bacterium with notable properties and significance in biotechnological applications. Biochemical studies suggest that...
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[NMR paper] Solution NMR studies of sterol carrier protein 2 like 2 (SCP2L2) reveal the insecticide specific structural characteristics of SCP2 proteins in Aedes Aegypti mosquitoes.
Solution NMR studies of sterol carrier protein 2 like 2 (SCP2L2) reveal the insecticide specific structural characteristics of SCP2 proteins in Aedes Aegypti mosquitoes.
Related Articles Solution NMR studies of sterol carrier protein 2 like 2 (SCP2L2) reveal the insecticide specific structural characteristics of SCP2 proteins in Aedes Aegypti mosquitoes.
Biochemistry. 2016 Aug 10;
Authors: Singarapu KK, Ahuja A, Potula PR, Ummanni R
Abstract
Sterol carrier protein 2 like 2 from Aedes aegypri (AeSCP2L2) plays an important role in...
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[NMR paper] The Tail-End Is Only the Beginning: NMR Study Reveals a Membrane-Bound State of BCL-XL.
The Tail-End Is Only the Beginning: NMR Study Reveals a Membrane-Bound State of BCL-XL.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The Tail-End Is Only the Beginning: NMR Study Reveals a Membrane-Bound State of BCL-XL.
J Mol Biol. 2015 Jul 3;427(13):2257-61
Authors: Hill RB, MacKenzie KR, Harwig MC
PMID: 25896456
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Orientation selective DEER measurements on vinculin tail at X-band frequencies reveal spin label orientations
Orientation selective DEER measurements on vinculin tail at X-band frequencies reveal spin label orientations
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 216</br>
Christoph Abé, Daniel Klose, Franziska Dietrich, Wolfgang H. Ziegler, Yevhen Polyhach, Gunnar Jeschke, Heinz-Jürgen Steinhoff</br>
Double electron electron resonance (DEER) spectroscopy has been established as a valuable method to determine distances between spin labels bound to protein molecules. Caused by selective excitation of molecular orientations DEER primary data also...
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Orientation Selective DEER Measurements on Vinculin Tail at X-Band Frequencies Reveal Spin Label Orientations
Orientation Selective DEER Measurements on Vinculin Tail at X-Band Frequencies Reveal Spin Label Orientations
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 8 January 2012</br>
Christoph*Abé, Daniel*Klose, Franziska*Dietrich, Wolfgang H.*Ziegler, Yevhen*Polyhach, ...</br>
Double electron electron resonance (DEER) spectroscopy has been established as a valuable method to determine distances between spin labels bound to protein molecules. Caused by selective excitation of molecular orientations DEER primary data also depend on the mutual orientation of...
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01-10-2012 03:38 PM
NMR, biophysical and biochemical studies reveal the minimal calmodulin-binding domain of the HIV-1 matrix protein.
NMR, biophysical and biochemical studies reveal the minimal calmodulin-binding domain of the HIV-1 matrix protein.
NMR, biophysical and biochemical studies reveal the minimal calmodulin-binding domain of the HIV-1 matrix protein.
J Biol Chem. 2011 Jul 28;
Authors: Samal AB, Ghanam RH, Fernandez TF, Monroe EB, Saad JS
Subcellular distribution of Calmodulin (CaM) in human immunodeficiency virus type-1 (HIV-1) infected cells is distinct from that observed in uninfected cells. CaM has been shown to interact and co-localize with the HIV-1 Gag protein...
Biochemical and NMR studies reveal specific interaction between STIMATE C-tail and PI(4,5)P(2) or PI(3,4,5)P(3)-containing membrane
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