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Default A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport prot

A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport protein.

Related Articles A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport protein.

Biophys Chem. 1991 Jul;40(3):329-37

Authors: Price WS, Kuchel PW, Cornell BA

Band 3, the erythrocyte anion transport protein, mediates the one-for-one exchange of bicarbonate and chloride ions across the membrane and consequently plays an important role in respiration. Binding to the protein forms the first step in the translocation of the chloride across the membrane. 35Cl and 37Cl NMR relaxation measurements at various field strengths were used to study chloride binding to the protein in the presence and absence of the transport inhibitor 4,4'-dinitrostilbene-2,2'-disulfonate. Significant differences occurred in the NMR relaxation rates depending on whether the inhibitor was present or not. The results indicate that the rate of chloride association and dissociation at each external binding site occurs on a time scale of less than or equal to 5 microseconds. This implies that the transmembrane flux is not limited by the rate of chloride binding to the external chloride binding site of band 3. The rotational correlation-time of chloride bound to band 3 was found to be greater than 20 ns with a quadrupole coupling constant of approximately 2 MHz.

PMID: 1912291 [PubMed - indexed for MEDLINE]



Source: PubMed
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