Normal and clofibrate-induced rat liver peroxisomes were studied in order to correlate the drug-elicited modifications of membrane permeability and fluidity with changes of the phospholipid component. The phospholipid composition and membrane fluidity of purified peroxisomal fractions from control and test animals were evaluated by 31P-NMR and fluorescence polarization spectroscopic techniques. The ultrastructural, enzymatic and electrophoretic analyses confirmed that the drug was able to induce: i) peroxisome proliferation; ii) increase of the catalase and fatty acyl CoA beta-oxidation system (FAOS) activities, and iii) neosynthesis of the 69 kDa peroxisomal membrane protein (PMP). The distribution pattern of the peroxisomal enzymes and the fluorescence polarization values of anilinonaphthalene-8-sulfonate (ANS)-labelled peroxisomes, suggests that the membrane permeability and fluidity are altered, while 31P-NMR spectroscopy seems to indicate that the phospholipid composition and the phospholipid/lysophospholipid ratio do not vary between test and control samples.
A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: Th
A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: The 1H-14N HSQC Experiment
Jiezhen Mao, Ling Jiang, Bin Jiang, Maili Liu and Xi-an Mao
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107745g/aop/images/medium/ja-2010-07745g_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja107745g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/3IJa7rhbOjw
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11-20-2010 06:29 AM
[NMR paper] Galactose metabolism in normal human lymphoblasts studied by (1)H, (13)C and (31)P NM
Galactose metabolism in normal human lymphoblasts studied by (1)H, (13)C and (31)P NMR spectroscopy of extracts.
Related Articles Galactose metabolism in normal human lymphoblasts studied by (1)H, (13)C and (31)P NMR spectroscopy of extracts.
NMR Biomed. 2001 May;14(3):192-8
Authors: Wehrli SL, Reynolds R, Chen J, Yager C, Segal S
The development of tools to follow and quantitate the fate of galactose in mammalian cells is crucial to the study and understanding of the inherited disorders of galactose metabolism. In this study we incubated...
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11-19-2010 08:32 PM
[NMR paper] The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/H
The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/HNF1 transcription factor.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/HNF1 transcription factor.
J Mol Biol. 1997 Apr 4;267(3):673-83
Authors: Schott O, Billeter M, Leiting B, Wider G, Wüthrich K
The nuclear magnetic resonance (NMR) solution structure of the non-classical homeodomain from the rat...
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08-22-2010 03:31 PM
[NMR paper] The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/H
The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/HNF1 transcription factor.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The NMR solution structure of the non-classical homeodomain from the rat liver LFB1/HNF1 transcription factor.
J Mol Biol. 1997 Apr 4;267(3):673-83
Authors: Schott O, Billeter M, Leiting B, Wider G, Wüthrich K
The nuclear magnetic resonance (NMR) solution structure of the non-classical homeodomain from the rat...
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08-22-2010 03:03 PM
[NMR paper] NMR relaxation data of water proton in normal tissues.
NMR relaxation data of water proton in normal tissues.
Related Articles NMR relaxation data of water proton in normal tissues.
Physiol Chem Phys Med NMR. 1996;28(4):205-38
Authors: Akber SF
The spin lattice relaxation time (T1) and spin spin relaxation time (T2) of water protons of normal human and animal tissues are archived to up-date those already published. The mechanisms for water proton relaxation times of tissues are reviewed with reference to water content, paramagnetic ion, protein dynamics content and organ weight.
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[NMR paper] Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclo
Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclophosphamide metabolites: determination of selective properties of DNA-interstrand and DNA-protein cross-linking mechanisms (alkaline elution and 31P-NMR-spectroscopy) and some aspects of the combination drug and radiotherapy.
Related Articles Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclophosphamide metabolites: determination of selective properties of DNA-interstrand and DNA-protein cross-linking mechanisms (alkaline elution and 31P-NMR-spectroscopy) and...
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08-21-2010 10:48 PM
[NMR paper] The roles of Glu-327 and His-446 in the bisphosphatase reaction of rat liver 6-phosph
The roles of Glu-327 and His-446 in the bisphosphatase reaction of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase probed by NMR spectroscopic and mutational analyses of the enzyme in the transient phosphohistidine intermediate complex.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles The roles of Glu-327 and His-446 in the bisphosphatase reaction of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase probed by NMR spectroscopic and mutational analyses of the enzyme in the transient phosphohistidine...
31P-NMR of liver peroxisome membranes from normal and clofibrate-treated rats.
Linear Mode
