NMR paper ?2 -Adrenergic Receptor Activation by Agonists Studied with (19) F NMR Spectroscopy.

		BioNMR > Educational resources > Journal club
[NMR paper] ?2 -Adrenergic Receptor Activation by Agonists Studied with (19) F NMR Spectroscopy.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2013, 08:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,188

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

?2 -Adrenergic Receptor Activation by Agonists Studied with (19) F NMR Spectroscopy.

?2 -Adrenergic Receptor Activation by Agonists Studied with (19) F NMR Spectroscopy.

?2 -Adrenergic Receptor Activation by Agonists Studied with (19) F NMR Spectroscopy.

Angew Chem Int Ed Engl. 2013 Aug 16;

Authors: Horst R, Liu JJ, Stevens RC, Wüthrich K

Abstract
Proteins in slow motion: (19) F NMR studies indicate that equilibria between active and inactive states of the human ?2 -adrenergic receptor require extensive structural rearrangements (arrows in picture). This was shown by an enthalpy difference of ?Ho ?40 kJ mol(-1) and a slow exchange rate, with kex

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] The Interaction between tRNA(Lys) 3 and the Primer Activation Signal Deciphered by NMR Spectroscopy. The Interaction between tRNA(Lys) 3 and the Primer Activation Signal Deciphered by NMR Spectroscopy. Related Articles The Interaction between tRNA(Lys) 3 and the Primer Activation Signal Deciphered by NMR Spectroscopy. PLoS One. 2013;8(6):e64700 Authors: Sleiman D, Barraud P, Brachet F, Tisne C Abstract The initiation of reverse transcription of the human immunodeficiency virus type 1 (HIV-1) requires the opening of the three-dimensional structure of the primer tRNA(Lys) 3 for its annealing to the viral RNA at the primer binding site...	nmrlearner	Journal club	0	06-14-2013 07:31 PM
[NMR paper] G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. Related Articles G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. Biochem J. 2013 Apr 15;451(2):343 Authors: Ding X, Zhao X, Watts A Abstract	nmrlearner	Journal club	0	03-29-2013 07:52 PM
[NMR paper] G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. Biochem J. 2013 Mar 15;450(3):443-57 Authors: Ding X, Zhao X, Watts A Abstract GPCRs (G-protein-coupled receptors) are versatile signalling molecules at the cell surface and make up the largest and most diverse family of membrane receptors in the human genome. They convert a large variety of extracellular stimuli into...	nmrlearner	Journal club	0	03-01-2013 09:57 PM
The interaction of cannabinoid receptor agonists, CP55940 and WIN55212-2 with membranes using solid state 2H NMR. The interaction of cannabinoid receptor agonists, CP55940 and WIN55212-2 with membranes using solid state 2H NMR. The interaction of cannabinoid receptor agonists, CP55940 and WIN55212-2 with membranes using solid state 2H NMR. Biochim Biophys Acta. 2011 Sep;1808(9):2095-101 Authors: Tian X, Pavlopoulos S, Yang DP, Makriyannis A Abstract Two key commonly used cannabinergic agonists, CP55940 and WIN55212-2, are investigated for their effects on the lipid membrane bilayer using (2)H solid state NMR, and the results are compared with our...	nmrlearner	Journal club	0	09-13-2011 08:27 PM
[NMR paper] Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study. Related Articles Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study. Biochim Biophys Acta. 2004 May 27;1663(1-2):74-81 Authors: Katragadda M, Maciejewski MW, Yeagle PL The recently reported crystal structure of bovine rhodopsin revealed a cytoplasmic helix (helix 8) in addition to the seven transmembrane helices. This domain is roughly perpendicular to the transmembrane bundle in the presence of an...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: e NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix. Related Articles NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix. Biochemistry. 2002 Mar 19;41(11):3596-604 Authors: Chung DA, Zuiderweg ER, Fowler CB, Soyer OS, Mosberg HI, Neubig RR A major, unresolved question in signal transduction by G protein coupled receptors (GPCRs) is to understand how, at atomic resolution, a GPCR activates a G protein. A...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Dynamic activation of protein function: a view emerging from NMR spectroscopy. Dynamic activation of protein function: a view emerging from NMR spectroscopy. Related Articles Dynamic activation of protein function: a view emerging from NMR spectroscopy. Nat Struct Biol. 2001 Nov;8(11):926-31 Authors: Wand AJ Recent developments in solution NMR methods have allowed for an unprecedented view of protein dynamics. Current insights into the nature of protein dynamics and their potential influence on protein structure, stability and function are reviewed. Particular emphasis is placed on the potential of fast side chain motion...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] 19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by 19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by Al3+ and F-. Related Articles 19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by Al3+ and F-. J Biol Chem. 1991 Feb 25;266(6):3396-401 Authors: Higashijima T, Graziano MP, Suga H, Kainosho M, Gilman AG 19F and 31P NMR spectroscopy was used to study the mechanism of activation of the alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) by Al3+, Mg2+, and F-. 19F NMR spectra of solutions containing Al3+,...	nmrlearner	Journal club	0	08-21-2010 11:16 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off