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Default 19F NMR as a versatile tool to study (membrane) protein structure and dynamics.

19F NMR as a versatile tool to study (membrane) protein structure and dynamics.

Related Articles 19F NMR as a versatile tool to study (membrane) protein structure and dynamics.

Biol Chem. 2018 Dec 01;:

Authors: Rose-Sperling D, Tran MA, Lauth LM, Goretzki B, Hellmich UA

Abstract
To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or GPCRs, the incorporation of a single reporter at a select site can significantly simplify the observables and the measurement/analysis requirements. Here we present examples using 19F NMR spectroscopy as a powerful, yet relatively straightforward tool to study (membrane) protein structure, dynamics and ligand interactions. We summarize methods to incorporate 19F labels into proteins and discuss the type of information that can be readily obtained for membrane proteins already from relatively simple NMR spectra with a focus on GPCRs as the membrane protein family most extensively studied by this technique. In the future, these approaches may be of particular interest also for many proteins that undergo complex functional dynamics and/or contain unstructured regions and thus are not amenable to X-ray crystallography or cryo electron microscopy studies.


PMID: 31004560 [PubMed - as supplied by publisher]



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