[ASAP] Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins

		BioNMR > Educational resources > Journal club
[ASAP] Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-18-2022, 11:26 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,191

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins

[ASAP] Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins

Feng-Jie Wu, Pascal S. Rieder, Layara Akemi Abiko, Philip Ro?ßler, Alvar D. Gossert, Daniel Ha?ussinger, and Stephan Grzesiek

Journal of the American Chemical Society
DOI: 10.1021/jacs.2c09692

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Magic angle spinning NMR of G protein-coupled receptors Magic angle spinning NMR of G protein-coupled receptors G protein-coupled receptors (GPCRs) have a simple seven transmembrane helix architecture which has evolved to recognize a diverse number of chemical signals. The more than 800 GPCRs encoded in the human genome function as receptors for vision, smell and taste, and mediate key physiological processes. Consequently, these receptors are a major target for pharmaceuticals. Protein crystallography and electron cryo-microscopy have provided high resolution structures of many GPCRs in both active and... More...	nmrlearner	Journal club	0	03-18-2022 12:01 AM
[NMR paper] Universality of Sodium Ion Binding Mechanism in Class A G-Protein-Coupled Receptors Universality of Sodium Ion Binding Mechanism in Class A G-Protein-Coupled Receptors The allosteric modulation of GPCRs by sodium (Na+) ions has drawn significant attention due to the availability of crystal structures of several receptors showing Na+ ions bound to the inactive conformations at the conserved Asp2.50. To date, structures from 24 families of GPCRs have been determined, though mechanistic insights into Na+ binding to the allosteric site are limited. We performed 100's of ?s long simulations of 15 GPCRs and elucidated their Na+ binding mechanism. In class A GPCRs, Na+ ion...	nmrlearner	Journal club	0	02-06-2018 07:42 PM
When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00845/20171016/images/medium/bi-2017-00845a_0003.gif Biochemistry DOI: 10.1021/acs.biochem.7b00845 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/4Eup-rPKLl8 More...	nmrlearner	Journal club	0	10-17-2017 10:19 AM
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee <img alt="" height="1" width="1" /> Global G-Protein Coupled Receptors (GPCRs) Industry Sacramento Bee NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ... G-PROTEIN COUPLED RECEPTORS (GPCR) - A CONCEPTUAL OVERVIEW II-7GPCR: Gateways to Cell II-7Classification of GPCRs II-7Table 1: List of Identified Known and Orphan (Non-Sensory)GPCRs ... and more » Read here	nmrlearner	Online News	0	08-15-2012 08:54 AM
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee <img alt="" height="1" width="1" /> Global G-Protein Coupled Receptors (GPCRs) Industry Sacramento Bee This report analyzes the Global market for G-Protein Coupled Receptors (GPCRs) in ... NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ... Read here	nmrlearner	Online News	0	06-13-2012 11:34 AM
Structure and function of G protein-coupled receptors using NMR spectroscopy Structure and function of G protein-coupled receptors using NMR spectroscopy Publication year: 2010 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 57, Issue 2</br> Joseph A. Goncalves, Shivani Ahuja, Sina Erfani, Markus Eilers, Steven O. Smith</br> </br> </br></br>	nmrlearner	Journal club	0	03-09-2012 09:16 AM
[NMR paper] Peptide hormone binding to G-protein-coupled receptors: structural characterization v Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques. Related Articles Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques. Med Res Rev. 2001 Sep;21(5):450-71 Authors: Mierke DF, Giragossian C G-protein-coupled receptors (GPCRs) allow cells to respond to calcium, hormones, and neurotransmitters. Not surprisingly, they currently make up the largest family of validated drug targets. Rational drug design for molecular regulators targeting GPCRs...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
Structure and function of G protein-coupled receptors using NMR spectroscopy Structure and function of G protein-coupled receptors using NMR spectroscopy Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 12 May 2010</br> Joseph A., Goncalves , Shivani, Ahuja , Sina, Erfani , Markus, Eilers , Steven O., Smith</br> More...	nmrlearner	Journal club	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off