[ASAP] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

		BioNMR > Educational resources > Journal club
[ASAP] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-02-2021, 06:00 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,188

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

[ASAP] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

Xiaoyan Wang?, Dongsheng Liu?, Ling Shen?, Fahui Li, Yongze Li, Lingyun Yang?, Tiandan Xu?, Houchao Tao?, Deqiang Yao?, Lijie Wu?, Kunio Hirata?, Laura M. Bohn, Alexandros Makriyannis×, Xiaohong Liu, Tian Hua?, Zhi-Jie Liu?, and Jiangyun Wang

Journal of the American Chemical Society
DOI: 10.1021/jacs.1c06847

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. Related Articles DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. Nat Commun. 2020 Sep 25;11(1):4857 Authors: Liu Q, He QT, Lyu X, Yang F, Zhu ZL, Xiao P, Yang Z, Zhang F, Yang ZY, Wang XY, Sun P, Wang QW, Qu CX, Gong Z, Lin JY, Xu Z, Song SL, Huang SM, Guo SC, Han MJ, Zhu KK, Chen X, Kahsai AW, Xiao KH, Kong W, Li FH, Ruan...	nmrlearner	Journal club	0	09-28-2020 09:16 PM
[ASAP] Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor Matthew T. Eddy, Zhan-Guo Gao, Philip Mannes, Nilkanth Patel, Kenneth A. Jacobson, Vsevolod Katritch, Raymond C. Stevens, Kurt Wüthrich https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b03805/20180620/images/medium/ja-2018-03805n_0006.gif Journal of the American Chemical Society DOI: 10.1021/jacs.8b03805 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	06-21-2018 09:01 AM
[NMR paper] A genetically encoded 19F NMR probe for lysine acetylation. A genetically encoded 19F NMR probe for lysine acetylation. A genetically encoded 19F NMR probe for lysine acetylation. Chem Commun (Camb). 2018 Mar 29;: Authors: Zhang F, Zhou Q, Yang G, An L, Li F, Wang J Abstract Advances in acetylated protein-protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has...	nmrlearner	Journal club	0	03-30-2018 06:40 PM
[NMR paper] Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent. Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent. Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent. Chem Sci. 2017 Nov 01;8(11):7631-7636 Authors: Roose BW, Zemerov SD, Dmochowski IJ Abstract Genetically encoded magnetic resonance imaging (MRI) contrast agents enable non-invasive detection of specific biomarkers in vivo. Here, we employed the hyper-CEST 129Xe NMR technique to quantify maltose (32 nM to 1 mM) through its modulation of conformational...	nmrlearner	Journal club	0	03-24-2018 12:21 PM
[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells. A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells. Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells. Angew Chem Int Ed Engl. 2016 Jun 15; Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ Abstract Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...	nmrlearner	Journal club	0	06-16-2016 12:06 PM
[NMR paper] Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor. Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor. Backbone NMR reveals allosteric signal transduction networks in the ?1-adrenergic receptor. Nature. 2016 Feb 3; Authors: Isogai S, Deupi X, Opitz C, Heydenreich FM, Tsai CJ, Brueckner F, Schertler GF, Veprintsev DB, Grzesiek S Abstract G protein-coupled receptors (GPCRs) are physiologically important transmembrane signalling proteins that trigger intracellular responses upon binding of extracellular ligands. Despite recent breakthroughs...	nmrlearner	Journal club	0	02-04-2016 11:46 AM
[NMR paper] A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation. A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation. A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation. Angew Chem Int Ed Engl. 2013 Feb 28; Authors: Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W, Tian C, Wang J Abstract Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein-protein interactions. The highly efficient genetic incorporation of...	nmrlearner	Journal club	0	03-02-2013 11:45 AM
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid. Related Articles Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid. Angew Chem Int Ed Engl. 2010 Nov 25; Authors: Nguyen TH, Ozawa K, Stanton-Cook M, Barrow R, Huber T, Otting G	nmrlearner	Journal club	0	11-27-2010 02:45 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off