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[NMR paper] NMR Metabolomics Investigates the Influence of Flavonoid-Enriched Rations on Chicken Plasma.
Jun 07, 2017 - 1:35 PM - by nmrlearner
nmrlearner's Avatar NMR Metabolomics Investigates the Influence of Flavonoid-Enriched Rations on Chicken Plasma.

Related Articles NMR Metabolomics Investigates the Influence of Flavonoid-Enriched Rations on Chicken Plasma.

J AOAC Int. 2017 Mar 01;100(2):315-322

Authors: Fotakis C, Lantzouraki DZ, Goliomytis M, Simitzis PE, Charismiadou M, Deligeorgis SG, Zoumpoulakis P

Abstract
The use of flavonoids as dietary supplements is well established, mainly due to their intense antioxidant and anti-inflammatory properties. In the present study, hesperidin, naringin, and vitamin E were used as additives at different concentrations in poultry rations in order to achieve meat of improved quality. NMR metabolomics was applied to chicken blood serum samples to discern whether and how the enriched rations affected the animals' metabolic profile. Variations in the metabolic patterns according to sustenance consumption were traced by orthogonal projections to latent structures discriminant analysis... [Read More]
0 Replies | 29 Views
[NMR paper] Assessment of dietary exposure and effect in humans: The role of NMR.
Jun 07, 2017 - 1:35 PM - by nmrlearner
nmrlearner's Avatar Assessment of dietary exposure and effect in humans: The role of NMR.

Related Articles Assessment of dietary exposure and effect in humans: The role of NMR.

Prog Nucl Magn Reson Spectrosc. 2016 08;96:58-72

Authors: van Duynhoven JP, Jacobs DM

Abstract
In human nutritional science progress has always depended strongly on analytical measurements for establishing relationships between diet and health. This field has undergone significant changes as a result of the development of NMR and mass spectrometry methods for large scale detection, identification and quantification of metabolites in body fluids. This has allowed systematic studies of the metabolic fingerprints that biological processes leave behind, and has become the research field of metabolomics. As a metabolic profiling technique, NMR is at its best when its unbiased nature, linearity and reproducibility are exploited in well-controlled nutritional intervention and cross-sectional population screening studies. Although its sensitivity is less good than that of mass... [Read More]
0 Replies | 42 Views
[NMR paper] Allosteric Sensing of Fatty Acid Binding by NMR: Application to Human Serum Albumin.
Jun 07, 2017 - 1:35 PM - by nmrlearner
nmrlearner's Avatar Allosteric Sensing of Fatty Acid Binding by NMR: Application to Human Serum Albumin.

Related Articles Allosteric Sensing of Fatty Acid Binding by NMR: Application to Human Serum Albumin.

J Med Chem. 2016 Aug 25;59(16):7457-65

Authors: Jafari N, Ahmed R, Gloyd M, Bloomfield J, Britz-McKibbin P, Melacini G

Abstract
Human serum albumin (HSA) serves not only as a physiological oncotic pressure regulator and a ligand carrier but also as a biomarker for pathologies ranging from ischemia to diabetes. Moreover, HSA is a biopharmaceutical with a growing repertoire of putative clinical applications from hypovolemia to Alzheimer's disease. A key determinant of the physiological, diagnostic, and therapeutic functions of HSA is the amount of long chain fatty acids (LCFAs) bound to HSA. Here, we propose to utilize (13)C-oleic acid for the NMR-based assessment of albumin-bound LCFA concentration (CONFA). (13)C-Oleic acid primes HSA for a LCFA-dependent allosteric transition that modulates the frequency separation between the two main (13)C NMR peaks of HSA-bound oleic acid... [Read More]
0 Replies | 44 Views
Surface-selective direct 17O DNP NMR of CeO2 nanoparticles #DNPNMR
Jun 05, 2017 - 3:59 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Surface-selective direct 17O DNP NMR of CeO2 nanoparticles #DNPNMR

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Hope, M.A., et al., Surface-selective direct 17O DNP NMR of CeO2 nanoparticles. Chem Commun (Camb), 2017. 53(13): p. 2142-2145.


https://www.ncbi.nlm.nih.gov/pubmed/28134945


Surface-selective direct 17O DNP has been demonstrated for the first time on CeO2 nanoparticles, for which the first three layers can be distinguished with high selectivity. Polarisation build-up curves show that the polarisation of the (sub-)surface sites builds up faster than the bulk, accounting for the remarkable surface selectivity.
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Go to The DNP-NMR Blog for more info.
0 Replies | 51 Views
A tailored multi-frequency EPR approach to accurately determine the magnetic resonance parameters of dynamic nuclear polarization agents: application to AMUPol #DNPNMR
Jun 05, 2017 - 3:59 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

A tailored multi-frequency EPR approach to accurately determine the magnetic resonance parameters of dynamic nuclear polarization agents: application to AMUPol #DNPNMR

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This is a very nice article illustrating the importance of understanding the EPR parameters of a polarizing agent used in DNP-NMR spectroscopy. Here the 9, 95 and 275 GHz EPR spectroscopy is used to characterize AMUPol and predict its performance in high-field DNP.






Gast, P., et al., A tailored multi-frequency EPR approach to accurately determine the magnetic resonance parameters of dynamic nuclear polarization agents: application to AMUPol. Phys. Chem. Chem. Phys., 2017. 19(5): p. 3777-3781.


https://www.ncbi.nlm.nih.gov/pubmed/28098267


To understand the dynamic nuclear polarization (DNP) enhancements of biradical polarizing agents, the magnetic resonance parameters need to be known. We describe a tailored EPR approach to accurately determine electron spin-spin coupling parameters using a combination of standard (9 GHz), high (95 GHz) and ultra-high (275 GHz) frequency EPR. Comparing liquid- and frozen-solution continuous-wave EPR spectra provides accurate anisotropic dipolar interaction D and isotropic exchange interaction J parameters of the DNP biradical AMUPol. We found that D was larger by as much as 30% compared to earlier estimates, and that J is 43 MHz, whereas before it was considered to be negligible. With the refined data, quantum... [Read More]
0 Replies | 66 Views
[NMR] PhD position in solid-state NMR of paramagnetic materials
Jun 05, 2017 - 3:59 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

[NMR] PhD position in solid-state NMR of paramagnetic materials

A PhD position is available in the group of Andrew Pell at the Department of Materials Environmental and Environmental Chemistry (MMK), Stockholm University (Sweden), in solid-state NMR of paramagnetic materials.
Closing date: August 7, 2017.


Project description
The aim of our research is to develop solid-state nuclear magnetic resonance (NMR) methods in order to allow a more accurate characterization, at the atomic scale, of the structure and dynamics of increasingly complex materials that are relevant in modern material science and chemistry. Specifically we focus on systems that contain paramagnetic metal ions, and how these ions dictate the properties of technologically important materials such as batteries, catalysts, and solid-state lighting phosphors.


Solid-state NMR is the method of choice for studying local structure and dynamics. However many interesting paramagnetic materials are, for technical reasons, beyond the ability of the current state of the art in solid-state NMR to study. This PhD project is therefore focussed on developing and specifically tailoring the techniques and capabilities of solid-state NMR for the analysis of samples containing these metal ions; for characterizing their presence, activity, role, and function in a range of different materials. Specifically the PhD student will develop new pulse schemes for exciting and detecting the NMR signals from quadrupolar nuclei (such as 2H, 14N, 23Na, 25Al, …) in paramagnetic materials, and incorporate these new schemes into more sophisticated experiments in order to separate the information from different spin interactions, which can then be... [Read More]
0 Replies | 57 Views
[NMR paper] Recent advances in measuring the kinetics of biomolecules by NMR relaxation dispersion spectroscopy.
Jun 05, 2017 - 3:02 AM - by nmrlearner
nmrlearner's Avatar Recent advances in measuring the kinetics of biomolecules by NMR relaxation dispersion spectroscopy.

Related Articles Recent advances in measuring the kinetics of biomolecules by NMR relaxation dispersion spectroscopy.

Arch Biochem Biophys. 2017 May 30;:

Authors: Ban D, Smith CA, de Groot BL, Griesinger C, Lee D

Abstract
Protein function can be modulated or dictated by the amplitude and timescale of biomolecular motion, therefore it is imperative to study protein dynamics. Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique capable of studying timescales of motion that range from those faster than molecular reorientation on the picosecond timescale to those that occur in real-time. Across this entire regime, NMR observables can report on the amplitude of atomic motion, and the kinetics of atomic motion can be ascertained with a wide variety of experimental techniques from real-time to milliseconds and several nanoseconds to picoseconds. Still a four orders of magnitude window between several nanoseconds and tens of microseconds has remained elusive. Here, we highlight new relaxation dispersion NMR techniques that serve to cover this "hidden-time" window up to hundreds of nanoseconds that achieve atomic resolution while studying the molecule under physiological conditions.


PMID: 28576576 [PubMed - as supplied by publisher]


... [Read More]
0 Replies | 162 Views
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