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[ASAP] Disulfide-Bond-Induced Structural Frustration and Dynamic Disorder in a Peroxiredoxin from MAS NMR
May 05, 2023 - 10:31 AM - by nmrlearner
nmrlearner's Avatar [ASAP] Disulfide-Bond-Induced Structural Frustration and Dynamic Disorder in a Peroxiredoxin from MAS NMR

Laura Troussicot, Alicia Vallet, Mikael Molin, Bjo?rn M. Burmann, and Paul Schanda



Journal of the American Chemical Society
DOI: 10.1021/jacs.3c01200



Source: Journal of the American Chemical Society
0 Replies | 415 Views
Ligand and G-protein selectivity in the ?-opioid receptor - Nature.com
May 05, 2023 - 10:31 AM - by nmrlearner
nmrlearner's Avatar Ligand and G-protein selectivity in the ?-opioid receptor - Nature.com

Ligand and G-protein selectivity in the ?-opioid receptor Nature.com Read here
0 Replies | 145 Views
Protein Crystallization Market Research Report 2023-2031 Market ... - The Northwestern Examiner
May 05, 2023 - 10:31 AM - by nmrlearner
nmrlearner's Avatar Protein Crystallization Market Research Report 2023-2031 Market ... - The Northwestern Examiner

Protein Crystallization Market Research Report 2023-2031 Market ... The Northwestern Examiner Read here
0 Replies | 156 Views
[NMR paper] Backbone and ILV side-chain methyl NMR resonance assignments of human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes
May 04, 2023 - 6:57 AM - by nmrlearner
nmrlearner's Avatar Backbone and ILV side-chain methyl NMR resonance assignments of human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes

Rev7 is a versatile HORMA (Hop1, Rev7, Mad2) family adaptor protein with multiple roles in mitotic regulation and DNA damage response, and an essential accessory subunit of the translesion synthesis (TLS) DNA polymerase Pol? employed in replication of damaged DNA. Within Pol?, the two copies of Rev7 interact with the two Rev7-bonding motifs (RBM1 and RBM2) of the catalytic subunit Rev3 by a mechanism characteristic of HORMA proteins whereby the "safety-belt" loop of Rev7 closes on the top of the...

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0 Replies | 204 Views
[NMR paper] Cholesterol Biases the Conformational Landscape of the Chemokine Receptor CCR3: A MAS SSNMR-Filtered Molecular Dynamics Study
May 04, 2023 - 6:57 AM - by nmrlearner
nmrlearner's Avatar Cholesterol Biases the Conformational Landscape of the Chemokine Receptor CCR3: A MAS SSNMR-Filtered Molecular Dynamics Study

Cholesterol directs the pathway of ligand-induced G protein-coupled receptor (GPCR) signal transduction. The GPCR C-C motif chemokine receptor 3 (CCR3) is the principal chemotactic receptor for eosinophils, with roles in cancer metastasis and autoinflammatory conditions. Recently, we discovered a direct correlation between bilayer cholesterol and increased agonist-triggered CCR3 signal transduction. However, the allosteric molecular mechanism escalating ligand affinity and G protein coupling is...

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0 Replies | 164 Views
[NMR paper] Quantitative Paramagnetic NMR-Based Analysis of Protein Orientational Dynamics on Membranes: Dissecting the KRas4B-Membrane Interactions
Apr 29, 2023 - 4:50 AM - by nmrlearner
nmrlearner's Avatar Quantitative Paramagnetic NMR-Based Analysis of Protein Orientational Dynamics on Membranes: Dissecting the KRas4B-Membrane Interactions

Peripheral membrane proteins can adopt distinct orientations on the surfaces of lipid bilayers that are often short-lived and challenging to characterize by conventional experimental methods. Here we describe a robust approach for mapping protein orientational landscapes through quantitative interpretation of paramagnetic relaxation enhancement (PRE) data arising from membrane mimetics with spin-labeled lipids. Theoretical analysis, followed by experimental verification, reveals insights into...

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0 Replies | 190 Views
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