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Quantification of glycosylated acute-phase proteins via NMR spectroscopy - John Wiley
Dec 23, 2023 - 11:47 PM - by nmrlearner
nmrlearner's Avatar Quantification of glycosylated acute-phase proteins via NMR spectroscopy - John Wiley

Quantification of glycosylated acute-phase proteins via NMR spectroscopy John Wiley Read here
0 Replies | 193 Views
[NMR paper] Enzymatic Peptide and Protein Bromination: The BromoTrp Tag
Dec 23, 2023 - 3:46 AM - by nmrlearner
nmrlearner's Avatar Enzymatic Peptide and Protein Bromination: The BromoTrp Tag


Angewandte Chemie International Edition, EarlyView.

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0 Replies | 179 Views
[NMR paper] Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR
Dec 22, 2023 - 6:21 AM - by nmrlearner
nmrlearner's Avatar Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR

Helical structures in proteins include not only ?-helices but also 3(10) and ? helices. These secondary structures differ in the registry of the C?O···H-N hydrogen bonds, which are i to i + 4 for ?-helices, i to i + 3 for 3(10) helices, and i to i + 5 for ?-helices. The standard NMR observable of protein secondary structures are chemical shifts, which are, however, insensitive to the precise type of helices. Here, we introduce a three-dimensional (3D) ¹H-detected experiment that measures and...

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Unlocking intrinsically disordered proteins: an interview with Peter Wright - News-Medical.Net
Dec 21, 2023 - 3:08 PM - by nmrlearner
nmrlearner's Avatar Unlocking intrinsically disordered proteins: an interview with Peter Wright - News-Medical.Net

Unlocking intrinsically disordered proteins: an interview with Peter Wright News-Medical.Net Read here
0 Replies | 88 Views
Activation of caspase-9 on the apoptosome as studied by methyl-TROSY NMR
Dec 21, 2023 - 3:08 PM - by nmrlearner
nmrlearner's Avatar Activation of caspase-9 on the apoptosome as studied by methyl-TROSY NMR

Alexander I. M. SeverT. Reid AldersonEnrico RennellaJames M. AraminiZi Hao LiuRobert W. HarknessLewis E. KayaDepartment of Chemistry, University of Toronto, Toronto, ON M5S 3H6, CanadabProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON M5G 0A4, CanadacDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, CanadadDepartment of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada...
Date:

Proceedings of the National Academy of Sciences, Volume 120, Issue 51, December 2023.
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PNAS:
Number: 51
Volume: 120
0 Replies | 117 Views
[NMR paper] Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches
Dec 21, 2023 - 3:08 PM - by nmrlearner
nmrlearner's Avatar Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches

The higher order structure (HOS) of monoclonal antibodies (mAbs) is an important quality attribute with strong contribution to clinically relevant biological functions and drug safety. Due to the multi-faceted nature of HOS, the synergy of multiple complementary analytical approaches can substantially improve the understanding, accuracy, and resolution of HOS characterization. In this study, we applied one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) spectroscopy coupled...

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[NMR paper] Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms
Dec 21, 2023 - 3:08 PM - by nmrlearner
nmrlearner's Avatar Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms

Intravenous gallium nitrate therapy is a novel therapeutic strategy deployed to combat chronic Pseudomonas aeruginosa biofilm infections in the lungs of cystic fibrosis (CF) patients by interfering with iron (Fe^(3+)) uptake. The therapy is a source of Ga^(3+), which competes with Fe^(3+) for siderophore binding, subsequently disrupting iron metabolism and inhibiting biofilm proliferation in vivo. It was recently demonstrated that the Pseudomonas quinolone signal (PQS) can chelate Fe^(3+) to...

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0 Replies | 128 Views
[NMR paper] The NMR signature of maltose-based glycation in full-length proteins
Dec 21, 2023 - 3:08 PM - by nmrlearner
nmrlearner's Avatar The NMR signature of maltose-based glycation in full-length proteins

Reducing sugars can spontaneously react with free amines in protein side chains leading to posttranslational modifications (PTMs) called glycation. In contrast to glycosylation, glycation is a non-enzymatic modification with consequences on the overall charge, solubility, aggregation susceptibility and functionality of a protein. Glycation is a critical quality attribute of therapeutic monoclonal antibodies. In addition to glucose, also disaccharides like maltose can form glycation products. We...

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