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Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses Nature.com Read here
0 Replies | 95 Views
[NMR paper] Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning

Deuterium rotating frame solid-state NMR relaxation measurements (²H R(1?)) are important tools in quantitative studies of molecular dynamics. We demonstrate how ²H to ^(13)C cross-polarization (CP) approaches under 10-40 kHz magic angle spinning rates can be combined with the ²H R(1?) blocks to allow for extension of deuterium rotating frame relaxation studies to methyl groups in biomolecules. This extension permits detection on the ^(13)C nuclei and, hence, for the achievement of site-specific...

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0 Replies | 101 Views
Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry - Nature.com
Feb 28, 2024 - 9:53 PM - by nmrlearner
nmrlearner's Avatar Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry - Nature.com

Interactions between the protein barnase and co-solutes studied by NMR | Communications Chemistry Nature.com Read here
0 Replies | 74 Views
Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com
Feb 28, 2024 - 9:53 PM - by nmrlearner
nmrlearner's Avatar Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com

Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid ScienceDirect.com Read here
0 Replies | 81 Views
NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science
Feb 28, 2024 - 9:40 AM - by nmrlearner
nmrlearner's Avatar NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science

NMR Structure Determination for Larger Proteins Using Backbone-Only Data Science Read here
0 Replies | 94 Views
[NMR paper] XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures
Feb 27, 2024 - 9:30 PM - by nmrlearner
nmrlearner's Avatar XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures

The combination of X-ray free-electron lasers (XFELs) with serial femtosecond crystallography represents cutting-edge technology in structural biology, allowing the study of enzyme reactions and dynamics in real time through the generation of `molecular movies'. This technology combines short and precise high-energy X-ray exposure to a stream of protein microcrystals. Here, the XFEL structure of carbonic anhydrase II, a ubiquitous enzyme responsible for the interconversion of CO(2) and...

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0 Replies | 210 Views
Protein structure determination... blindfolded - Nature.com
Feb 27, 2024 - 9:20 AM - by nmrlearner
nmrlearner's Avatar Protein structure determination... blindfolded - Nature.com

Protein structure determination... blindfolded Nature.com Read here
0 Replies | 85 Views
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