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	[NMR paper] Deciphering the Therapeutic Role of Lactate in Combating Disuse-Induced Muscle Atrophy: An NMR-Based Metabolomic Study in Mice Deciphering the Therapeutic Role of Lactate in Combating Disuse-Induced Muscle Atrophy: An NMR-Based Metabolomic Study in Mice Disuse muscle atrophy (DMA) is a significant healthcare challenge characterized by progressive loss of muscle mass and function resulting from prolonged inactivity. The development of effective strategies for muscle recovery is essential. In this study, we established a DMA mouse model through hindlimb suspension to evaluate the therapeutic potential of lactate in alleviating the detrimental effects on the gastrocnemius muscle. Using NMR-based metabolomic analysis,... nmrlearner	Today 05:00 PM by nmrlearner	0	5	Journal club
	Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight Abstract A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium for NMR studies. Using this protocol, the D2O and 2H-glucose content of the media and protonation level of ILV labeling precursors (ketobutyrate and ketovalerate) were varied. The relaxation rate of the multiple-quantum (MQ) state that is present during the HMQC-TROSY pulse sequence was measured for... nmrlearner	Today 05:00 PM by nmrlearner	0	15	Journal club
	[NMR paper] Efficient Segmental Isotope Labeling of Integral Membrane Proteins for High-Resolution NMR Studies Efficient Segmental Isotope Labeling of Integral Membrane Proteins for High-Resolution NMR Studies High-resolution structural NMR analyses of membrane proteins are challenging due to their large size, resulting in broad resonances and strong signal overlap. Among the isotope labeling methods that can remedy this situation, segmental isotope labeling is a suitable strategy to simplify NMR spectra and retain high-resolution structural information. However, protein ligation within integral membrane proteins is complicated since the hydrophobic protein fragments are insoluble, and the removal... nmrlearner	Today 04:52 AM by nmrlearner	0	45	Journal club
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