Forum: NMR Questions and Answers
11-15-2006, 04:41 PM
|
Replies: 3
Views: 8,620
Was some effort made to allow amide exchange to...
Was some effort made to allow amide exchange to occur, ie unfolding and refolding in H2O, before you ran your spectra? If not you may not have enough amide protons there for HN experiments to work. ...
|
Forum: NMR Questions and Answers
11-13-2006, 04:27 PM
|
Replies: 3
Views: 8,620
The CBCA(CO)NH experiment depends on an initial...
The CBCA(CO)NH experiment depends on an initial 1H-13C transfer if your protein is deuterated you have no 1H's to excite on the carbons so you will get no signal, when you have a deuterated protein...
|