In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of...
[NMR paper] Isotope labeling for studying RNA by solid-state NMR spectroscopy.
Isotope labeling for studying RNA by solid-state NMR spectroscopy.
Related Articles Isotope labeling for studying RNA by solid-state NMR spectroscopy.
J Biomol NMR. 2018 Apr 12;:
Authors: Marchanka A, Kreutz C, Carlomagno T
Abstract
Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state nuclear magnetic resonance spectroscopy (ssNMR) provides a unique...
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04-14-2018 01:49 PM
Isotope labeling for studying RNA by solid-state NMR spectroscopy
Isotope labeling for studying RNA by solid-state NMR spectroscopy
Abstract
Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state nuclear magnetic resonance spectroscopy (ssNMR) provides a unique opportunity to study large biomolecules in a non-crystalline state at atomic resolution. Application of ssNMR to RNA, however, is still at an early stage of development and presents...
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04-12-2018 01:11 PM
[NMR paper] Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
J Struct Biol. 2017 Nov 29;:
Authors: Pinto C, Mance D, Julien M, Daniels M, Weingarth M, Baldus M
Abstract
Significant progress has been made in obtaining a structural insight into the assembly of the ?-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane...
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12-05-2017 07:35 PM
[NMR paper] Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Prog Nucl Magn Reson Spectrosc. 2016 Aug;96:1-46
Authors: Schanda P, Ernst M
Abstract
Magic-angle spinning solid-state NMR spectroscopy is an important technique to study molecular structure, dynamics and interactions, and is rapidly gaining importance in biomolecular sciences. Here we provide an...
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04-26-2016 12:14 PM
Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules
Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules
Publication date: August 2016
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 96</br>
Author(s): Paul Schanda, Matthias Ernst</br>
Magic-angle spinning solid-state NMR spectroscopy is an important technique to study molecular structure, dynamics and interactions, and is rapidly gaining importance in biomolecular sciences. Here we provide an overview of experimental approaches to study molecular dynamics by MAS solid-state NMR,...
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04-09-2016 03:54 AM
[NMR paper] Solid-state 31P NMR investigation on the status of guanine nucleotides in paclitaxel-stabilized microtubules.
Solid-state 31P NMR investigation on the status of guanine nucleotides in paclitaxel-stabilized microtubules.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Solid-state 31P NMR investigation on the status of guanine nucleotides in paclitaxel-stabilized microtubules.
Magn Reson Chem. 2015 May;53(5):330-6
Authors: Lee GH, Oh SY, Yeo KJ, Ferdous T, Cho M, Paik Y
Abstract
Microtubule dynamics is a target for many chemotherapeutic...
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01-09-2016 04:06 PM
Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Angew Chem Int Ed Engl. 2010 Oct 4;49(41):7504-7
Authors: Kumar A, Heise H, Blommers MJ, Krastel P, Schmitt E, Petersen F, Jeganathan S, Mandelkow EM, Carlomagno T, Griesinger C, Baldus M
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03-13-2011 04:01 AM
[NMR paper] Solid-state NMR approaches for studying membrane protein structure.
Solid-state NMR approaches for studying membrane protein structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--arjournals.annualreviews.org-images-AnnualReviews100x25.gif Related Articles Solid-state NMR approaches for studying membrane protein structure.
Annu Rev Biophys Biomol Struct. 1992;21:25-47
Authors: Smith SO, Peersen OB
Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins
Linear Mode
