Quantifying the thermodynamics of protein unfolding using 2D NMR spectroscopy
 

Quantifying the thermodynamics of protein unfolding using 2D NMR spectroscopy

A topic that has attracted considerable interest in recent years is the possibility to perform thermodynamic studies of proteins directly in-cell or in complex environments which mimic the cellular interior. Nuclear magnetic resonance (NMR) could be an attractive technique for these studies but its applicability has so far been limited by technical issues. Here, we demonstrate that 2D NMR methods can be successfully applied to measure thermodynamic parameters provided that a suitable choice of...

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