NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
Nucleic Acids Res. 2010 Nov 1;38(21):7814-21
Authors: Cevec M, Thibaudeau C, Plavec J
We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state NMR spectroscopy. let-7 miRNAs control the timing of development of the nematode Caenorhabditis elegans and are highly conserved in mammals. The sequence and structure of the two conserved let-7 complementary sites, LCS1 and LCS2, in the 3'-untranslated region of lin-41 mRNA are important for a proper downregulation of lin-41. The high-resolution NMR structure reveals details of the binding of let-7 miRNA to lin-41 mRNA which involves formation of a complex with non-canonical structural elements within the seed region. LCS1co exhibits a stem-loop structure with two stems, an asymmetric internal loop and an adenine bulge. Comparison with the NMR solution-state structure of the let-7:lin-41 complex involving the LCS2-binding site shows that conformational freedom of the asymmetric internal loop of LCS1co correlates with a smaller bend between the upper and lower stems in comparison to the well-defined asymmetric loop of LCS2co.
Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.
Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.
Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.
J Struct Funct Genomics. 2011 Jul 23;
Authors: Eletsky A, Ruyechan WT, Xiao R, Acton TB, Montelione GT, Szyperski T
The solution NMR structure of protein MED25(391-543), comprising the activator interacting domain (ACID) of subunit 25 of the human mediator, is presented along with the measurement of...
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Solution structure of the human Tax-interacting protein-1
Solution structure of the human Tax-interacting protein-1
Solution structure of the human Tax-interacting protein-1
Content Type Journal Article
Pages 329-334
DOI 10.1007/s10858-009-9361-8
Authors
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01-09-2011 12:46 PM
[NMR paper] Joint X-ray and NMR refinement of the yeast L30e-mRNA complex.
Joint X-ray and NMR refinement of the yeast L30e-mRNA complex.
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Structure. 2004 Jul;12(7):1165-76
Authors: Chao JA, Williamson JR
L30e, a Saccharomyces cervisiae ribosomal protein, regulates its own expression by binding to a purine-rich asymmetric internal loop located in both its pre-mRNA and mature mRNA. A crystal structure of an MBP-L30e fusion protein in complex with an RNA containing the pre-mRNA regulatory site was solved at 3.24 A. Interestingly, the...
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11-24-2010 09:51 PM
[NMR paper] NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock pr
NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: implications for KaiA-KaiC interaction.
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Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1479-84
Authors: Vakonakis I, Sun J, Wu T, Holzenburg A, Golden SS, LiWang AC
KaiA is a two-domain circadian clock protein in cyanobacteria, acting as the positive element in a feedback loop that sustains the oscillation....
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11-24-2010 09:25 PM
[NMR paper] NMR structure of conserved eukaryotic protein ZK652.3 from C. elegans: a ubiquitin-li
NMR structure of conserved eukaryotic protein ZK652.3 from C. elegans: a ubiquitin-like fold.
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Proteins. 2002 Sep 1;48(4):733-6
Authors: Cort JR, Chiang Y, Zheng D, Montelione GT, Kennedy MA
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11-24-2010 08:58 PM
[NMR paper] NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop bin
NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein.
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RNA. 2002 Jan;8(1):83-96
Authors: DeJong ES, Marzluff WF, Nikonowicz EP
The 3' end of replication-dependent histone mRNAs terminate in a conserved sequence containing a stem-loop. This 26-nt sequence is the binding site for a protein, stem-loop binding protein (SLBP), that is involved in multiple aspects of histone mRNA metabolism...
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11-24-2010 08:49 PM
[NMR paper] Mapping the cytochrome c553 interacting site using 1H and 15N NMR.
Mapping the cytochrome c553 interacting site using 1H and 15N NMR.
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FEBS Lett. 1999 Oct 22;460(1):77-80
Authors: Morelli X, Guerlesquin F
Cytochrome c553 is the electron transfer partner of formate dehydrogenase and of
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[NMR paper] 1H-NMR studies on association of mRNA cap-analogues with tryptophan-containing peptid
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Biochim Biophys Acta. 1996 Mar 7;1293(1):97-105
Authors: Stolarski R, Sitek A, StepiÅ?ski J, Jankowska M, Oksman P, Temeriusz A, Darzynkiewicz E, Lönnberg H, Shugar D
1H-NMR spectroscopy was applied to a study of the mode of interaction, in aqueous medium in the pH range 5.2-8.5 and at low and high temperatures, between several mono- and dinucleotide...
NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
Hybrid Mode
