A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle [Biophysics and Computational Biology]
 

A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle [Biophysics and Computational Biology]

Gili Abramov, Algirdas Velyvis, Enrico Rennella, Leo E. Wong, Lewis E. Kay...
Date: 2020-06-09

The development of methyl-transverse relaxation-optimized spectroscopy (methyl-TROSY)–based NMR methods, in concert with robust strategies for incorporation of methyl-group probes of structure and dynamics into the protein of interest, has facilitated quantitative studies of high-molecular-weight protein complexes. Here we develop a one-pot in vitro reaction for producing NMR quantities of methyl-labeled... Read More


PNAS:
Number: 23
Volume: 117