A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle [Biophysics and Computational Biology]
A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle [Biophysics and Computational Biology]
Gili Abramov, Algirdas Velyvis, Enrico Rennella, Leo E. Wong, Lewis E. Kay...
Date: 2020-06-09
The development of methyl-transverse relaxation-optimized spectroscopy (methyl-TROSY)–based NMR methods, in concert with robust strategies for incorporation of methyl-group probes of structure and dynamics into the protein of interest, has facilitated quantitative studies of high-molecular-weight protein complexes. Here we develop a one-pot in vitro reaction for producing NMR quantities of methyl-labeled... Read More
PNAS:
Number: 23
Volume: 117
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[NMR paper] A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle.
A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle.
Related Articles A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle.
Proc Natl Acad Sci U S A. 2020 May 26;:
Authors: Abramov G, Velyvis A, Rennella E, Wong LE, Kay LE
Abstract
The development of methyl-transverse relaxation-optimized spectroscopy (methyl-TROSY)-based NMR methods, in concert with robust strategies for incorporation of methyl-group...
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05-29-2020 04:59 PM
Exploring long-range cooperativity in the 20S proteasome core particle from Thermoplasma acidophilum using methyl-TROSY-based NMR [Biophysics and Computational Biology]
Exploring long-range cooperativity in the 20S proteasome core particle from Thermoplasma acidophilum using methyl-TROSY-based NMR
Enrico Rennella, Rui Huang, Zanlin Yu, Lewis E. Kay...
Date: 2020-03-10
The 20S core particle (CP) proteasome is a molecular assembly catalyzing the degradation of misfolded proteins or proteins no longer required for function. It is composed of four stacked heptameric rings that form a barrel-like structure, sequestering proteolytic sites inside its lumen. Proteasome function is regulated by gates derived from... Read More
PNAS:
Number: 10
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03-16-2020 04:59 PM
Probing the cooperativity of Thermoplasma acidophilum proteasome core particle gating by NMR spectroscopy [Biophysics and Computational Biology]
Probing the cooperativity of Thermoplasma acidophilum proteasome core particle gating by NMR spectroscopy
Rui Huang, Felipe Perez, Lewis E. Kay...
Date: 2017-11-14
The 20S proteasome core particle (20S CP) plays an integral role in cellular homeostasis by degrading proteins no longer required for function. The process is, in part, controlled via gating residues localized to the ends of the heptameric barrel-like CP structure that occlude substrate entry pores, preventing unregulated degradation of... Read More
PNAS:
Number: 46
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11-15-2017 08:36 AM
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
<img alt="" height="1" width="1">
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins
SelectScience
Labeling schemes commonly employed for NMR investigations of high-molecular-weight proteins utilize selective incorporation of protons and 13C isotopes into methyl groups of Ileδ1, Leuδ and Valγ side-chains in a highly deuterated environment (commonly ...
Read here
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08-09-2017 09:26 AM
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
<img alt="" height="1" width="1">
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins
SelectScience
Labeling schemes commonly employed for NMR investigations of high-molecular-weight proteins utilize selective incorporation of protons and 13C isotopes into methyl groups of Ileδ1, Leuδ and Valγ side-chains in a highly deuterated environment (commonly ...
Read here
nmrlearner
Online News
0
08-08-2017 10:10 AM
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
<img alt="" height="1" width="1">
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins
SelectScience
Labeling schemes commonly employed for NMR investigations of high-molecular-weight proteins utilize selective incorporation of protons and 13C isotopes into methyl groups of Ileδ1, Leuδ and Valγ side-chains in a highly deuterated environment (commonly ...
Read here
nmrlearner
Online News
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08-07-2017 07:31 PM
Methyl-TROSY NMR studies of proteasome allostery [Biophysics and Computational Biology]
Methyl-TROSY NMR studies of proteasome allostery
Ruschak, A. M., Kay, L. E....
Date: 2012-12-11
Protein degradation plays a critical role in cellular homeostasis, in regulating the cell cycle, and in the generation of peptides that are used in the immune response. The 20S proteasome core particle (CP), a barrel-like structure consisting of four heptameric protein rings stacked axially on top of each other, is... Read More
PNAS:
Number: 50
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12-12-2012 08:19 AM
Architecture of the high mobility group nucleosomal protein 2-nucleosome complex as revealed by methyl-based NMR [Biophysics and Computational Biology]
Architecture of the high mobility group nucleosomal protein 2-nucleosome complex as revealed by methyl-based NMR
Kato, H., van Ingen, H., Zhou, B.-R., Feng, H., Bustin, M., Kay, L. E., Bai, Y....
Date: 2011-07-26
Chromatin structure and function are regulated by numerous proteins through specific binding to nucleosomes. The structural basis of many of these interactions is unknown, as in the case of the high mobility group nucleosomal (HMGN) protein family that regulates various chromatin functions, including transcription. Here, we report the architecture of the HMGN2-nucleosome...
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A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle [Biophysics and Computational Biology]
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