Identification of protein surfaces by NMR measurements with a pramagnetic Gd(III) che
 

Identification of protein surfaces by NMR measurements with a pramagnetic Gd(III) chelate.

Related Articles Identification of protein surfaces by NMR measurements with a pramagnetic Gd(III) chelate.

J Am Chem Soc. 2002 Jan 23;124(3):372-3

Authors: Pintacuda G, Otting G

Gd-diethylenetriamine pentaacetic acid-bismethylamide, Gd(DTPA-BMA), is shown to be a reagent suitable for the identification of protein surfaces. Compared to the conventionally used spin-label TEMPOL, Gd(DTPA-BMA) is a stronger relaxation agent, requiring lesser concentrations to achieve the same paramagnetic relaxation enhancement of solvent-exposed protein protons. It is also less hydrophobic and therefore less prone to specific binding to proteins. Relaxation enhancements predicted by a second-sphere interaction model correlated with experimental data recorded with ubiquitin, while the correlation with corresponding data recorded with TEMPOL was poor.

PMID: 11792196 [PubMed - indexed for MEDLINE]



Source: PubMed