NMR paper Two-Dimensional NMR Spectroscopy of the G Protein-Coupled Receptor A2AAR in Lipid Nanodiscs

		BioNMR > Educational resources > Journal club
[NMR paper] Two-Dimensional NMR Spectroscopy of the G Protein-Coupled Receptor A2AAR in Lipid Nanodiscs

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-30-2023, 09:47 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,211

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Two-Dimensional NMR Spectroscopy of the G Protein-Coupled Receptor A2AAR in Lipid Nanodiscs

Two-Dimensional NMR Spectroscopy of the G Protein-Coupled Receptor A2AAR in Lipid Nanodiscs

Eight hundred and twenty-six human G protein-coupled receptors (GPCRs) mediate the actions of two-thirds of the human hormones and neurotransmitters and over one-third of clinically used drugs. Studying the structure and dynamics of human GPCRs in lipid bilayer environments resembling the native cell membrane milieu is of great interest as a basis for understanding structure-function relationships and thus benefits continued drug development. Here, we incorporate the human A(2A) adenosine...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Production of human A2AAR in lipid nanodiscs for 19F-NMR and single-molecule fluorescence spectroscopy Production of human A2AAR in lipid nanodiscs for 19F-NMR and single-molecule fluorescence spectroscopy We describe production of the human A(2A) adenosine receptor (A(2A)AR), a class A G protein-coupled receptor (GPCR) for ^(19)F-NMR and single-molecule fluorescence (SMF) spectroscopy. We explain in detail steps shared between the two sample preparation strategies, including expression and isolation of A(2A)AR and assembly of A(2A)AR in lipid nanodiscs and procedures for incorporation of either ^(19)F-NMR or fluorescence probes. Protocols for SMF experiments include sample setup, data... ...	nmrlearner	Journal club	0	07-17-2022 10:07 PM
Conformational dynamics of a G protein–coupled receptor helix 8 in lipid membranes - Science Advances Conformational dynamics of a G protein–coupled receptor helix 8 in lipid membranes - Science Advances Conformational dynamics of a G protein–coupled receptor helix 8 in lipid membranes Science Advances Read here	nmrlearner	Online News	0	08-15-2020 05:51 AM
[ASAP] Dynamic G Protein-Coupled Receptor Signaling Probed by Solution NMR Spectroscopy Dynamic G Protein-Coupled Receptor Signaling Probed by Solution NMR Spectroscopy https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00032/20200302/images/medium/bi0c00032_0014.gif Biochemistry DOI: 10.1021/acs.biochem.0c00032 http://feeds.feedburner.com/~r/acs/bichaw/~4/4bPBV3Rv0UE More...	nmrlearner	Journal club	0	03-03-2020 11:18 PM
[NMR paper] Exploring Lipid and Membrane Protein Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy. Exploring Lipid and Membrane Protein Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Exploring Lipid and Membrane Protein Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy. Methods Mol Biol. 2020;2127:397-419 Authors: Bibow S Abstract The relationship of membrane protein function and the surrounding lipid bilayer goes far beyond...	nmrlearner	Journal club	0	03-03-2020 11:18 PM
Receptor Activity Modifying Proteins Have LimitedEffects on the Class B G Protein-Coupled Receptor Calcitonin Receptor-LikeReceptor Stalk Receptor Activity Modifying Proteins Have LimitedEffects on the Class B G Protein-Coupled Receptor Calcitonin Receptor-LikeReceptor Stalk http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01180/20180207/images/medium/bi-2017-01180k_0008.gif Biochemistry DOI: 10.1021/acs.biochem.7b01180 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/8Z5smrN57aI More...	nmrlearner	Journal club	0	02-08-2018 04:02 AM
Effect of Lipid Composition on the Membrane Orientationof the G Protein-Coupled Receptor Kinase 2–G?1?2 Complex Effect of Lipid Composition on the Membrane Orientationof the G Protein-Coupled Receptor Kinase 2–G?1?2 Complex http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00354/20160506/images/medium/bi-2016-00354u_0005.gif Biochemistry DOI: 10.1021/acs.biochem.6b00354 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/uR48svftNQQ More...	nmrlearner	Journal club	0	05-07-2016 03:19 AM
[NMR paper] The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy. The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy. Chemistry. 2014 Mar 13; Authors: Schmidt P, Thomas L, Müller P, Scheidt HA, Huster D Abstract	nmrlearner	Journal club	0	03-14-2014 07:34 PM
[NMR paper] Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled r Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled receptor. Related Articles Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled receptor. J Am Chem Soc. 2005 Jun 8;127(22):8010-1 Authors: Tian C, Breyer RM, Kim HJ, Karra MD, Friedman DB, Karpay A, Sanders CR The seven-transmembrane-spanning G protein-coupled receptor (GPCR) superfamily plays many important roles in basic biology, human health, and human disease. Here, well-resolved solution NMR spectra are presented for a human...	nmrlearner	Journal club	0	11-25-2010 08:21 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off