Interferon-induced transmembrane protein 3 (IFITM3) is an antiviral transmembrane protein thought to serve as the primary factor for inhibiting replication of a large number of viruses, including the West Nile virus, the Dengue virus, the Ebola virus, and the Zika virus. Production of this 14.5 kDa, 133-residue, transmembrane protein, especially with essential posttranslational modifications by recombinant expression is challenging. In this report, we document the chemical synthesis of IFTIM3 in multimilligram quantities (>15 mg) and the preparation of phosphorylated and fluorescent variants. The synthesis was accomplished by KAHA ligations, which operate under acidic aqueous/organic mixtures that excel at solubilizing even the exceptionally hydrophobic C-terminal region of IFITM3. The synthetic material readily incorporates in model vesicles and forms the basis for using synthetic, homogenous IFITM3 and its derivatives for further studying its structure and biological mode of action.
[NMR paper] Palladium in Chemical Protein Synthesis and Modifications
Palladium in Chemical Protein Synthesis and Modifications
The field of site-specific modification of proteins has drawn significant attentions in recent years owing to its high importance in various research areas such as the development of novel therapeutics and understanding the biochemical and cellular behaviors of proteins. The presence of a large number of reactive functional groups in the protein of interest and in the cellular environment renders the particular modification at a specific site a highly challenging task. However, with the development of sophisticated...
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04-07-2017 02:01 AM
Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression
Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00488/20160721/images/medium/bi-2016-00488z_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00488
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07-22-2016 01:34 AM
[NMR paper] Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3.
Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3.
Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3.
Sci Rep. 2016;6:24029
Authors: Ling S, Zhang C, Wang W, Cai X, Yu L, Wu F, Zhang L, Tian C
Abstract
Interferon-inducible transmembrane protein IFITM3 was known to restrict the entry of a wide spectrum of viruses to the cytosol of the host. The mechanism used by the protein to restrict viral entry is unclear given the unavailability of the...
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04-06-2016 10:29 PM
[NMR paper] Synthesis, biological activity and NMR-based structural studies of deltorphin I analogues modified in message domain with a new ?,?-disubstituted glycines.
Synthesis, biological activity and NMR-based structural studies of deltorphin I analogues modified in message domain with a new ?,?-disubstituted glycines.
Related Articles Synthesis, biological activity and NMR-based structural studies of deltorphin I analogues modified in message domain with a new ?,?-disubstituted glycines.
Chem Biol Drug Des. 2016 Jan 25;
Authors: Lasota A, Fr?czak O, Muchowska A, Nowakowski M, Maciejczyk M, Ejchart A, Olma A
Abstract
This paper describes new deltorphin I analogues in which phenylalanine...
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01-26-2016 03:40 PM
[NMR paper] Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-? Variants and Structural Analysis by Solution NMR Spectroscopy.
Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-? Variants and Structural Analysis by Solution NMR Spectroscopy.
Related Articles Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-? Variants and Structural Analysis by Solution NMR Spectroscopy.
PLoS One. 2015;10(10):e0139710
Authors: Dammers C, Gremer L, Neudecker P, Demuth HU, Schwarten M, Willbold D
Abstract
Alzheimer's disease (AD) is the leading cause of dementia in the elderly and is...
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10-06-2015 10:39 PM
[NMR paper] Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Carbohydr Res. 2013 Nov 1;383C:1-13
Authors: Cui L, Ling CC, Sadowska J, Bundle DR
Abstract
A rat monoclonal antibody 9D4 raised against the cell surface N-glycan of the parasite Trichinella spirallis protects rats...
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11-20-2013 12:42 AM
[Ln(BH4)2(THF)2](Ln = Eu, Yb)?A Highly Luminescent Material. Synthesis, Properties,Reactivity, and NMR Studies
(Ln = Eu, Yb)?A Highly Luminescent Material. Synthesis, Properties,Reactivity, and NMR Studies
Sebastian Marks, Joachim G. Heck, Marija H. Habicht, Pascual On?a-Burgos, Claus Feldmann and Peter W. Roesky
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja308077t/aop/images/medium/ja-2012-08077t_0010.gif
Journal of the American Chemical Society
DOI: 10.1021/ja308077t
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10-10-2012 03:14 PM
Total Synthesis and StructureConfirmation of Elatenyne:Success of Computational Methods for NMR Prediction with Highly FlexibleDiastereomers
Total Synthesis and StructureConfirmation of Elatenyne:Success of Computational Methods for NMR Prediction with Highly FlexibleDiastereomers
Bryony S. Dyson, Jonathan W. Burton, Te-ik Sohn, Byungsook Kim, Hoon Bae and Deukjoon Kim
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja304554e/aop/images/medium/ja-2012-04554e_0011.gif
Journal of the American Chemical Society
DOI: 10.1021/ja304554e
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Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane Associated Protein IFITM3 and Modified Variants
Linear Mode
