(15)N and (13)C Group-Selective Techniques Extend the Scope of STD NMR Detection of Weak Host-Guest Interactions and Ligand Screening.
Related Articles (15)N and (13)C Group-Selective Techniques Extend the Scope of STD NMR Detection of Weak Host-Guest Interactions and Ligand Screening.
Chembiochem. 2010 Sep 28;
Authors: Kövér KE, Wéber E, Martinek TA, Monostori E, Batta G
Saturation transfer difference (STD) is a valuable tool for studying the binding of small molecules to large biomolecules and for obtaining detailed information on the binding epitopes. Here, we demonstrate that the proposed (15)N/(13)C variants of group-selective, "GS-STD" experiments provide a powerful approach to mapping the binding epitope of a ligand even in the absence of efficient spin diffusion within the target protein. Therefore, these experimental variants broaden the scope of STD studies to smaller and/or more-dynamic targets. The STD spectra obtained in four different experimental setups (selective (1)H STD, (15)N GS-STD, (13)C(Ar) and (13)C(aliphatic) GS-STD approaches) revealed that the signal-intensity pattern of the difference spectra is affected by both the type and the spatial distribution of the excited "transmitter" atoms, as well as by the efficiency of the spin-diffusion-mediated magnetization transfer. The performance of the experiments is demonstrated on a system by using the lectin, galectin-1 and its carbohydrate ligand, lactose.
PMID: 20878964 [PubMed - as supplied by publisher]
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PubMed