Fluctuations, exchange processes, and water diffusion in aqueous protein systems: A study of bovine serum albumin by diverse NMR techniques.
Related Articles Fluctuations, exchange processes, and water diffusion in aqueous protein systems: A study of bovine serum albumin by diverse NMR techniques.
Biophys J. 1990 Nov;58(5):1183-97
Authors: Kimmich R, Gneiting T, Kotitschke K, Schnur G
Experimental frequency, concentration, and temperature dependences of the deuteron relaxation times T(1) and T(2) of D(2)O solutions of bovine serum albumin are reported and theoretically described in a closed form without formal parameters. Crucial processes of the theoretical concept are material exchange, translational diffusion of water molecules on the rugged surfaces of proteins, and tumbling of the macromolecules. It is also concluded that, apart from averaging of the relaxation rates in the diverse deuteron phases, material exchange contributes to transverse relaxation by exchange modulation of the Larmor frequency. The rate limiting factor of macromolecular tumbling is determined by the free water content. In a certain analogy to the classical free-volume
theory, a "free-water-volume theory" is presented. There are two characteristic water mass fractions indicating the saturation of the hydration shells (C(s) approximately 0.3) and the onset of protein tumbling (C(0) approximately 0.6). The existence of the translational degrees of freedom of water molecules in the hydration shells has been verified by direct measurement of the diffusion coefficient using an NMR field-gradient technique. The concentration and temperature dependences show phenomena indicating a percolation transition of clusters of free water. The threshold water content was found to be C(c) (w) approximately 0.43.
PMID: 19431772 [PubMed]
Source:
PubMed