Thread: NMR paper High resolution NMR for screening ligand/protein binding.
View Single Post
  #1  
Unread 08-21-2010, 04:03 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,191
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default High resolution NMR for screening ligand/protein binding.
High resolution NMR for screening ligand/protein binding.

Related Articles High resolution NMR for screening ligand/protein binding.

Curr Opin Drug Discov Devel. 1999 Jul;2(4):396-400

Authors: Shapiro MJ, Wareing JR

High resolution NMR spectroscopy has provided a versatile tool for assessing ligand/receptor interactions. NMR-based methods are currently being investigated which may prove valuable for compound screening. These techniques include the use of chemical shift perturbations, the monitoring of translational diffusion and the observation of NOEs. Novel experiments like TROSY and NOE pumping will play significant roles in looking at proteins larger than 30 kDa. New probe technology such as the Cryoprobe could have a significant impact on NMR-based screening for drug discovery by lowering the necessary amount of (15)N-labled protein.

PMID: 19649968 [PubMed]



Source: PubMed
Reply With Quote

Did you find this post helpful? Yes | No