Rapid Determination of the Topology of Oligomeric ?-Helical Membrane Proteins by Water- and Lipid-Edited Methyl NMR
Single-span oligomeric ?-helical transmembrane proteins are common in virus ion channels, which are targets of antiviral drugs. Knowledge about the high-resolution structures of these oligomeric ?-helical bundles is so far scarce. Structure determination of these membrane proteins by solid-state NMR traditionally requires resolving and assigning protein chemical shifts and measuring many interhelical distances, which are time-consuming. To accelerate experimental structure determination, here we...
More...