Thread: NMR paper Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy
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Default Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy
Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy

In NMR spectroscopy, residual dipolar couplings (RDCs) have emerged as one of the most exquisite probes of biological structure and dynamics. The measurement of RDCs relies on the partial alignment of the molecule of interest, for example by using a liquid crystal as a solvent. Here, we establish bacterial type 1 pili as an alternative liquid-crystalline alignment medium for the measurement of RDCs. To achieve alignment at pilus concentrations that allow for efficient NMR sample preparation, we...

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