Qualitative and Quantitative Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy
Solution-state nuclear magnetic resonance (NMR) spectroscopy can be used to monitor protein-carbohydrate interactions. Two-dimensional ¹H-^(15)N heteronuclear single quantum coherence (HSQC)-based techniques described in this chapter can be used quickly and effectively to screen a set of possible carbohydrate-binding partners, to quantify the dissociation constant (K(d)) of any identified interactions, and to the map the carbohydrate-binding site on the structure of a protein. Here, we describe...
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