Thread: NMR paper Solution phase refinement of active site structure using 2D NMR and judiciously 13C-labeled cytochrome P450
View Single Post
  #1  
Unread 01-24-2023, 06:06 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,212
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default Solution phase refinement of active site structure using 2D NMR and judiciously 13C-labeled cytochrome P450
Solution phase refinement of active site structure using 2D NMR and judiciously 13C-labeled cytochrome P450

The Cytochrome P450 (CYP450) superfamily has been the subject of intense research for over six decades. Here the HU227 strain of E. coli, lacking the ?-aminolevulinic acid (?-ALA) synthase gene, was employed, along with [5-^(13)C] ?-ALA, in the heterologous expression of P450cam harboring a prosthetic group labeled with ^(13)C at the four methine carbons (C(m)) and pyrrole C(?) positions. The product was utilized as a proof of principle strategy for defining and refining solution phase active...

More...
Reply With Quote

Did you find this post helpful? Yes | No