Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces
Nuclear magnetic resonance (NMR) spectroscopy is a key method for determining the structural dynamics of proteins in their native solution state. However, the low sensitivity of NMR typically necessitates nonphysiologically high sample concentrations, which often limit the relevance of the recorded data. We show how to use hyperpolarized water by dissolution dynamic nuclear polarization (DDNP) to acquire protein spectra at concentrations of 1 ?M within seconds and with a high signal-to-noise...
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